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PCNA Mouse mAb

In stock

PCNA Mouse mAb

MSDS

COA

Stock Information

Item (SKU)	Specifications	Is In Stock
Ab155826-10μl (Trial Size) Apply for free trial size（?） The same customer can apply for trial packaging three times a year, while the same product can only apply for trial packaging once a year	10μl	In Stock
Ab155826-20μl	20μl	Out of Stock
Ab155826-50μl	50μL	In Stock
Ab155826-100μl	100μL	In Stock
Ab155826-1ml	1ml	In Stock

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Basic Information

Name	PCNA Mouse mAb
Specifications & Purity	mAb (3D7); Mouse anti Human PCNA Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
Host species	Mouse
Specificity	PCNA
Application	WB,Flow,ELISA,ICC/IF
Species reactivity（Reacts with)	Human,Mouse?,Rat?
Species reactivity（Predicted to work with)	Chicken,Cow,Dog,Pig,Rabbit
Immunogen	Full length recombinant human PCNA
Positive control	WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells
偶联标记	Unconjugated

Product Properties

Antibody Type	Primary antibody
克隆	Monoclonal
Clone	C04/3D7
Format	Whole IgG
Isotype	IgG1
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Source	Hybridoma supernatant
Form	Liquid
Storage buffer/Formulation	PBS, pH 7.2
Preservatives	No
Concentration	Lot to lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped in	Dry ice
Stability and Storage	Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze/thaw cycle.

Application

Application	Notes
WB	1/1,000-1/10,000 ;?Predicted molecular weight: 29 kDa.
Flow	0.016-2.0μg/mL
ICC/IF	0.5 μg/mL

Images

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution

Samples: Lysates at 20 μg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

ICC/IF : PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Ab155826) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor? 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Flow Cytometry
Flow analysis of PCNA (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. PCNA Mouse mAb (Ab155826) at 1/4000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Antibody (Ab155826) at 0.05 μg/mL
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 29.0 kDa
Observed band size: 32.0 kDa
Exposure time: 97.0 s?

Document Information

Quality Inspection Report COA

Please input Lot number:

SDS-CN SDS-EN

Specification Sheet

FAQs and Articles

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