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Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488)

Secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488)
规格或纯度: pAb; Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488); ICC, IF, IHC, Flow, ELISA
  • 应用: ELISA,IF,FLow
  • 亚型: IgG
有货

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab138205-100μl 100μL 期货 Stock Image
Ab138205-1ml 1ml 期货 Stock Image

基本信息

产品名称 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488)
规格或纯度 pAb; Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488); ICC, IF, IHC, Flow, ELISA
免疫原 Rabbit IgG

产品属性

克隆 Polyclonal
Format Whole IgG
亚型 IgG
纯化方法 Antigen Affinity Purified
物理外观 Liquid
储存缓冲液 Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
防腐剂 0.02% Sodium Azide
浓度 1 mg/mL
储存温度 -20°C储存
运输条件 超低温冰袋运输
稳定性与储存 Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

应用

Application Notes
ELISA

1/5000 - 1/20000

IF

1/100-1/1000

FLow

1/500-1/1000

图片

Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) (Ab138205) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified?PABPN1 Rabbit mAb (Ab119979)?at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (Alexa Fluor? 647) 1/1000. Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) (Ab138205) was used as the secondary antibody at 1/500 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

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