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研究领域
免疫学
免疫球蛋白
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Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647)

Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647)

Secondary antibody, Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647)

规格或纯度: pAb; Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647); Flow, IF, ICC

应用: IF/ICC,Flow
种属反应性: Rabbit?
亚型: IgG

有货

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab136882-100μg	100μg	现货
Ab136882-1mg	1mg	期货
Ab136882-500μg	500μg	现货

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基本信息

产品名称	Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647)
规格或纯度	pAb; Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647); Flow, IF, ICC
特异性	Rabbit IgG
反应种属	Rabbit?
免疫原	Native rabbit IgG
阳性对照	IF/ICC: Mouse cerebellum; Mouse brain; Human brain; Rat cerebellum; Rat brain

产品属性

克隆	Polyclonal
Format	Whole IgG
亚型	IgG
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10 mM TBS (pH=7.4) with 1% BSA, 0.03% ProClin300 and 50% Glycerol
防腐剂	0.03% ProClin300
浓度	2.0mg/ml
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles

应用

Application	Notes
IF/ICC	1/100-1/1000
Flow	1/100-1/1000

图片

IF: Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647) (Ab136882)

Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647) (Ab136882) - IF
IF analysis of HeLa cells labeling FXR1 with purified?Recombinant FXR1 Antibody (Ab104281)?at 1/1000 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution - Goat Anti-Mouse IgG H&L (Alexa Fluor? 488) 1/1000. Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647) (Ab136882) was used as the secondary antibody at 1/1000 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

IHC: Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647) (Ab136882)

Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647) (Ab136882) - IHC
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min.Blocking buffer (normal goat serum) at 37°C for 30min. Antibody incubation with (GFAP) polyclonal antibody at 1/200 overnight at 4°C, followed by a conjugated Donkey Anti-Rabbit IgG H&L (Alexa Fluor? 647) (Ab136882) for 90 minutes, and DAPI for nuclei staining.