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Antibodies
Secondary Antibodies
Unconjugated
Goat Anti-Human IgG Antibody

Goat Anti-Human IgG Antibody

In stock

Goat Anti-Human IgG Antibody

MSDS

COA

Stock Information

Item (SKU)	Specifications	Is In Stock
Ab170447-1mg	1mg	In Stock
Ab170447-5mg	5mg	In Stock
Ab170447-10mg	10mg	In Stock

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Basic Information

Name	Goat Anti-Human IgG Antibody
Specifications & Purity	≥ 95%
Application	ELISA,CLIA,LFAs,WB and other immunoassay methods

Product Properties

Isotype	Human IgG1
Purification Method	ntigen affinity chromatography
Purity	≥ 95%
Form	liquid
Storage buffer/Formulation	10mM phosphate buffered saline (PBS), pH7.4
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped in	Dry ice

Images

ELISA: Goat Anti-Human IgG Antibody (Ab170447)

Goat Anti-Human IgG Antibody (Ab170447) - ELISA
Goat Anti-Human IgG Antibody (Ab170447) was tested by ELISA. The plate was coated with different amounts of human IgG (Ab170213) (3-fold started from 1000 ng/mL. Goat Anti-Human IgG Antibody (Ab170447) was then added at a series of different dilution ratios. Rabbit-Anti-Goat IgG H&L HRP (Ab141534) was used at 1/10000 dilution as the secondary antibody.

ELISA: Goat Anti-Human IgG Antibody (Ab170447)

Goat Anti-Human IgG Antibody (Ab170447) - ELISA
Cross-reactivity of Goat Anti-Human IgG Antibody (Ab170447) was tested by ELISA. The wells were coated with the indicated IgG standards at 1 μg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Human IgG Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit-Anti-Goat IgG H&L HRP (Ab141534) was used at 1/10000 dilution as the secondary antibody.
For the batch tested, Goat Anti-Human IgG Antibody (Ab170447) showed a cross-reactivity with rabbit IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/8000.

Document Information

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SDS-CN SDS-EN

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