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内参抗体
PCNA Mouse mAb

PCNA Mouse mAb

Mouse monoclonal (3D7) to PCNA-Loading Control

规格或纯度: mAb (3D7); Mouse anti Human PCNA Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated

应用: WB,Flow,ICC/IF
种属反应性: Human,Mouse?,Rat?
亚型: IgG1

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货号 (SKU)	包装规格	是否现货
Ab155826-50μl	50μL	现货
Ab155826-100μl	100μL	现货
Ab155826-1ml	1ml	期货

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基本信息

产品名称	PCNA Mouse mAb
规格或纯度	mAb (3D7); Mouse anti Human PCNA Antibody; WB, Flow, ICC/IF, ELISA; Unconjugated
宿主	Mouse
特异性	PCNA
应用	WB,Flow,ELISA,ICC/IF
反应种属	Human,Mouse?,Rat?
预测反应种属	Chicken,Cow,Dog,Pig,Rabbit
免疫原	Full length recombinant human PCNA
阳性对照	WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells
偶联标记	Unconjugated

产品属性

抗体类型	Primary antibody
克隆	Monoclonal
克隆号	C04/3D7
Format	Whole IgG
亚型	IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
来源	Hybridoma supernatant
物理外观	Liquid
储存缓冲液	PBS, pH 7.2
防腐剂	No
浓度	Lot to lot
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze/thaw cycle.

应用

Application	Notes
WB	1/1,000-1/10,000 ;?Predicted molecular weight: 29 kDa.
Flow	0.016-2.0μg/mL
ICC/IF	0.5 μg/mL

图片

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution

Samples: Lysates at 20 μg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

ICC/IF : PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Ab155826) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor? 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Flow Cytometry
Flow analysis of PCNA (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. PCNA Mouse mAb (Ab155826) at 1/4000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.