| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab155823-50μl | 50μL |
现货  |
| |
| Ab155823-100μl | 100μL |
现货 |
| |
| Ab155823-1ml | 1ml |
期货 |
|
| 产品名称 | beta Tubulin Mouse mAb |
|---|---|
| 规格或纯度 | mAb (2F1); Mouse anti Human beta tubulin Antibody; WB, Flow, ICC / IF, ELISA; Unconjugated |
| 宿主 | Mouse |
| 特异性 | beta tubulin |
| 应用 | WB,Flow,ELISA,ICC/IF |
| 反应种属 | Human,Mouse?,Rat? |
| 预测反应种属 | Chicken,Cow,Dog,Pig,Rabbit |
| 免疫原 | Recombinant human beta tubulin |
| 阳性对照 | WB: HeLa, A431, HEK293, MCF7, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells |
| 偶联标记 | Unconjugated |
| 抗体类型 | Primary antibody |
|---|---|
| 克隆 | Monoclonal |
| 克隆号 | CD01/2F1 |
| Format | Whole IgG |
| 亚型 | IgG3 |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 来源 | Hybridoma supernatant |
| 物理外观 | Liquid |
| 储存缓冲液 | PBS, pH 7.2 |
| 防腐剂 | No |
| 浓度 | 0.8mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Application | Notes |
|---|---|
| Flow |
0.016-2.0μg/mL
|
| ICC/IF |
0.5 μg/mL
|
| WB |
1/2,000-1/100,000; Predicted molecular weight: 50 kDa
|
beta Tubulin Mouse mAb (Ab155823) - Western Blot
All lanes: beta Tubulin Mouse mAb (Ab155823) at 1/10000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 50 kDa
beta Tubulin Mouse mAb (Ab155823) - ICC/IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor? 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
beta Tubulin Mouse mAb (Ab155823) - Flow Cytometry
Flow analysis of beta Tubulin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
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