| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
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Ab098863-10μl (试用装) 申请此免费试用装（?） 作为尊贵的客户，您每年可免费申请三次您选择的不同货号试用装产品，尽享探索与享受！
|
10μl |
期货  |
| |
|
Ab098863-50μl |
50μL |
现货 |
| |
|
Ab098863-100μl |
100μL |
现货 |
| |
|
Ab098863-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant Cytokeratin 18 Antibody |
|---|---|
| 规格或纯度 | Recombinant (RR693); Rabbit anti Human Cytokeratin 18 Antibody; WB, IHC, Flow, IF/ICC, IP; Unconjugated |
| 宿主 | Rabbit |
| 特异性 | KRT18 |
| 应用 | WB,Flow,IHC,IP,IF/ICC |
| 反应种属 | Human,Rat? |
| 交叉反应 | Cross-reactivity with other species has not been measured. |
| 免疫原 | A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 18 was used as an immunogen. |
| 阳性对照 | WB: Hela, A431, HepG2, T47D, MCF-7, A549 cell lysate; Rat Liver. IHC: Colonic adenocarcinoma tissue. Flow: HCT-116 cells. ICC/IF: HCT-116 cells. IP: Hela whole cell lysate. |
| 偶联标记 | Unconjugated |
| 抗体类型 | Primary antibody |
|---|---|
| 克隆 | Recombinant |
| 克隆号 | RR693 |
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 来源 | CHO supernatant |
| 物理外观 | Liquid |
| 储存缓冲液 | 59% 1*PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3. |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.5mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20°C for 2 years. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| WB |
1/2000 - 1/5000
|
| IHC | 1/800 - 1/1600 |
| Flow | 1/800 - 1/2000 |
| IF/ICC | 1/800 - 1/2000 |
| IP | 1/25 |
| IHC |
1/800 - 1/1600
|
Recombinant Cytokeratin 18 Antibody (Ab098863) - Western Blot
All lanes: Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 44 kDa
Exposure time: 1.0 s
Recombinant Cytokeratin 18 Antibody (Ab098863) - ICC/IF
Recombinant Cytokeratin 18 Antibody (Ab098863) staining Cytokeratin 18 in HCT-116 cells by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1/2000) at 4°C. An Alexa Fluor? 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor? 488- conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant Cytokeratin 18 Antibody (Ab098863) - IHC
Immunohistochemistry analysis of colonic adenocarcinoma tissue labelling Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Cytokeratin 18 Antibody (Ab098863) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained Cytokeratin 18 with Recombinant Cytokeratin 18 Antibody (Ab098863) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (Ab098863, 1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor? 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant Cytokeratin 18 Antibody (Ab098863) - IP
Cytokeratin 18 was immunoprecipitated from 0.4mg of HeLa whole cell lysate with Recombinant Cytokeratin 18 Antibody (Ab098863) at 1/25 dilution.
Lane 1: Ab098863 IP in HeLa whole cell lysate
Lane 2: PBS instead of Ab098863 in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input)
Secondary: GAR HRP for IP 1:500
Exposure time: 20.0 s
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