| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab091004-50μl | 50μL |
现货  |
| |
| Ab091004-100μl | 100μL |
现货 |
| |
| Ab091004-1ml | 1ml |
期货 |
|
| 产品名称 | beta Actin Antibody |
|---|---|
| 规格或纯度 | pAb; Rabbit anti Human beta Actin Antibody; WB, Flow, IHC, IF, ICC; Unconjugated |
| 宿主 | Rabbit |
| 特异性 | ACTB |
| 应用 | WB,Flow,IF,ICC,IHC |
| 免疫原 | Synthetic MAP peptide derived from human beta-Actin: 1-200/375. |
| 阳性对照 | WB: NIH/3T3, C6, A549, Ramos, Jurkat, HeLa, MCF-7 lysates. IHC: Human cervical carcinoma tissue. ICC/IF: HeLa cells. |
| 偶联标记 | Unconjugated |
| 抗体类型 | Primary antibody |
|---|---|
| 克隆 | Polyclonal |
| Format | Whole IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 来源 | Serum |
| 物理外观 | Liquid |
| 储存缓冲液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% ProClin 300 and 50% Glycerol. |
| 防腐剂 | 0.03% ProClin 300 |
| 浓度 | 1.0mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20 °C for one year. Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| WB |
1:5000-50000
|
| IHC |
1:500-1000
|
| Flow |
1μg/Test
|
| ICC/IF |
1:100
|
beta Actin Antibody (Ab091004) - Western Blot
All lanes: beta Actin Antibody (Ab091004) at 1/50000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
beta Actin Antibody (Ab091004) - IHC
Tissue: Human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded.
Antigen retrieval: Citrate buffer (0.01M, pH 6.0) boiling bathing for 15 minutes; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37℃ for 20 minutes.
Incubation: beta Actin Antibody (Ab091004) 1/1500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
beta Actin Antibody (Ab091004) - ICC/IF
Hela cells: 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with beta Actin Antibody (Ab091004) 1/100, 90 minutes at 37°C; Followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI was used to stain the cell nuclei.
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