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Recombinant Human MIF Protein(Active)

规格或纯度: Animal Free, ≥95%(SDS-PAGE), Active, E.coli, No tag, 1-115 aa
  • 蛋白长度: Full length protein
  • 蛋白标签: No tag

  • 应用: Active,SDS-PAGE
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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp148808-10μg 10μg 现货 Stock Image
rp148808-50μg 50μg 现货 Stock Image
rp148808-100μg 100μg 现货 Stock Image
rp148808-1mg 1mg 期货 Stock Image
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基本描述

产品名称 Recombinant Human MIF Protein
规格或纯度 Animal Free, ≥95%(SDS-PAGE), Active, E.coli, No tag, 1-115 aa
产品介绍


Purity
≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

Function
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
生物活性 Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
种属 Human
氨基酸 1-115 aa
序列 MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA
蛋白标签 No tag
蛋白长度 Full length protein
Carrier Free Yes
AnimalFree Yes
Accession# P14174
来源 Recombinant
预测分子量 12.5 kDa
SDS-PAGE 12.5 kDa

产品规格参数

应用 Active,SDS-PAGE
物理外观 Lyophilized
储存缓冲液 PBS, pH 7.4 with 5% trehalose and 0.02% Tween-80
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

图片

Recombinant Human MIF protein (rp148808)-Protein Bioactivity
Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.

Recombinant Human MIF protein (rp148808)-SDS-PAGE
Recombinant Human MIF protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 12.5 kDa.

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