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Phaseolus vulgaris lectin M (PHA-M)
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基本描述
| 英文名称 | Phaseolus vulgaris lectin M (PHA-M) |
|---|---|
| 别名 | PHA; 植物血球凝集素(菜豆) |
| 生化机理 | PHA由两种分子种类组成，即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素（PHA-E），和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素（PHA-L）。 PHA-E不具有血型特异性，但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前，PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。 |
| 储存温度 | 2-8°C储存 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
Features ? Potent mitogen ? High erythroagglutinating activity Description PHA-M is the mucoprotein variant of phytohemaglutinin isolated from red kidney bean. It contains up to 20% carbohydrates in conjugation with the protein which presents two bands corresponding to about 35 kDa according to SDS-PAGE in presence of mercaptoethanol. It is a potent mitogen used to stimulate cell proliferation in lymphocyte cultures and also has a powerful erythroagglutinating activity. Storage and stability Stable for at least 3 years from production date when stored below -20 ?C. May be shipped at ambient temperature. After reconstitution in PBS pH 7.4, the solution may be stored frozen in working aliquots for up to 12 months. Repeated freez- ing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
化学和物理性质
| 溶解性 | PBS: 2 mg/mL |
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