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Phaseolus vulgaris lectin P (PHA-P), native isomer composition
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基本描述
| 英文名称 | Phaseolus vulgaris lectin P (PHA-P), native isomer composition |
|---|---|
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
Features? Ultrapure quality Agglutinates leucocytes and erythrocytes Mixture of L and E subunits Sugar specificity: complex oligosaccharides Lyophilized powder ? Product description Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containingL (leucoagglutinin) and E (erythroagglutinin) subunits incompositions L1E3, L2E2, L3E1, E4. Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides . PHA-P is not blood group specific and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml. PHA-P lectin is supplied as a white to light-pinkessentially salt-free lyophilized powder from 50 mM NH4HCO3,which evaporates during the lyophilization procedure. Thepurity of the lectin is determined by SDS-PAGE generatingone single band. Applications Lateral flow erythrocyte separation Typical subunit composition Total L subunit composition: 60-65% Total E subunit composition: 35-40% L4 25-35% L3E1 20-30% L2E2 10-20% L1E3 17-27% E4 1-11% Directions for use The lectin may be reconstituted in a suitable buffer for yourapplication. Shipping and storage The product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months. |
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