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应用
蛋白质生物学
Western Blotting
Recombinant Streptavidin Protein (TRITC)

Recombinant Streptavidin Protein (TRITC)

规格或纯度: Recombinant; Streptavidin (TRITC); WB, IF

有货

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
rp169049-100μg	100μg	现货
rp169049-1mg	1mg	现货

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基本描述

产品名称	Recombinant Streptavidin Protein (TRITC)
规格或纯度	Recombinant; Streptavidin (TRITC); WB, IF
产品介绍	Introduction: Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a dissociation constant on the order of ≈10?1? mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. TRITC conjugated streptavidin can be used to localize or to detect biotinylated biomolecules in cells, tissue sections or other fluorescent immunoassays. Recommended Usage for Application： IF: 1:1000 WB: 1:4000-1:30000 Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

产品规格参数

物理外观	Liquid
储存缓冲液	10mM PBS (pH 7.4) buffer containing 1% BSA, 50% glycerol, 0.05% Proclin
防腐剂	0.05% ProClin300
浓度	1.0 mg/mL
储存温度	避光,-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
CAS编号和信息	9013-20-1

安全信息

象形图	Harmful
信号词	Warning
危险声明	H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation
预防措施声明	P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319
WGK Germany	3
个人防护装备	Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

应用

Application	Notes
IF	1:1000
WB	1/4000-1/30000

图片

IF: Recombinant Streptavidin Protein (TRITC) (rp169049)

Recombinant Streptavidin Protein (TRITC) (rp169049) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Biotin) (Ab176033) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (TRITC) (rp169049) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

IF: Recombinant Streptavidin Protein (TRITC) (rp169049)

Recombinant Streptavidin Protein (TRITC) (rp169049) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (TRITC) (rp169049) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.