Recombinant Streptavidin Protein (Cy5) (rp169047) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.