| 产品名称 | HRP 标记的链霉亲和素 |
|---|---|
| 规格或纯度 | Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed. |
| 产品介绍 |
背景 链霉亲和素是一种来自streptomyces avidinii菌的非糖基化蛋白。链亲和素同源四聚体对生物素具有特别高的非共价结合亲和力。当与酶（如辣根过氧化物酶或碱性磷酸酶）形成偶联物并与比色或发光底物发育系统结合时，链霉亲和素与生物素化抗体在许多应用中得到广泛应用，包括Western blot、ELISA、ELISPOT、免疫细胞化学和免疫组化。 推荐工作浓度 WB : 1:10000-1:100000 ELISA: 1:1000-1:10000 避免使用含有生物素的稀释液，试剂不得含有叠氮化钠，叠氮化钠会抑制辣根过氧化物酶的活性。实际试验操作过程中可根据具体实验情况适当调整Streptavidin(HRP)的稀释比例。 Background Streptavidin is a non-glycosylated protein from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry. Recommended Usage： WB: 1:10000 -1:100000 ELISA: 1:1000-1:10000 Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application. |
| 种属 | Streptomyces avidinii |
| 序列 | MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLG STFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWT VAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVG HDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ |
| 蛋白长度 | Full length protein |
| Carrier Free | 1% BSA |
| 偶联标记 | HRP |
| 来源 | Native |
| 应用 | WB, Dot, ELISA, ICC, IHC-Fr, IHC-P |
|---|---|
| 物理外观 | Liquid |
| 储存缓冲液 | PBS (pH 7.4) buffer containing 1% BSA, glycerol, and 0.05% ProClin? 300 as a preservative. |
| 防腐剂 | 0.05% ProClin? 300 |
| 浓度 | Streptavidin Concentration: 1 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle and protected from prolonged exposure to light. Prepare working dilution on day of use. |
| Application | Notes |
|---|---|
| ELISA |
1/1000-1/10000
|
| WB | 1:10000-1:100000 |
WB-Streptavidin (HRP) (np156148)
Various whole cell lysates were subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:10000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 10s.
ELISA-Streptavidin (HRP) (np156148)
ELISA results of Streptavidin (HRP) (np156148) tested against Biotin-BSA. Each well was coated in duplicate with 1 ng of Biotin-BSA.
WB-Streptavidin (HRP) (np156148)
HT-29 whole cell lysate was subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:5000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 8s.
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