| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab170784-1mg | 1mg |
现货  |
| |
| Ab170784-2mg | 2mg |
现货 |
| |
| Ab170784-10mg | 10mg |
现货 |
|
| 产品名称 | Goat Anti-Chicken IgY H&L Antibody |
|---|---|
| 规格或纯度 | Secondary Antibody; Goat Anti-Chicken IgY H&L Antibody; WB, ELISA, CLIA, LF, GICA; Unconjugated |
| 宿主 | Goat |
| 特异性 | Chicken IgY |
| 应用 | WB,ELISA,CLIA,LF |
| 反应种属 | Chicken? |
| 交叉反应 | No cross reactivity with mouse IgG, rabbit IgG, and human IgG. |
| 免疫原 | Chicken IgY |
| 偶联标记 | Unconjugated |
| 抗体类型 | secondary antibody |
|---|---|
| 克隆 | Polyclonal |
| Format | Whole IgG |
| 亚型 | Goat IgG |
| SDS-PAGE | 150kDa |
| 纯化方法 | Antigen affinity purified |
| 来源 | Serum |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, pH 7.4 |
| 浓度 | 6.0 mg/ml |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| WB |
1/400 - 1/2000
|
| ELISA |
1/5000?- 1/15000
|
| LF |
0.5-1.0mg/ml
|
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - ELISA
Goat Anti-Chicken IgY H&L Antibody (Ab170784) was tested by direct ELISA. The plate was coated with different amounts of Chicken IgY Antibody (Ab00841). A serial dilution Goat Anti-Chicken IgY H&L Antibody (Ab170784) was then added at a series of different dilution ratios. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - ELISA
Cross-reactivity of Goat Anti-Chicken IgY H&L Antibody (Ab170784) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 μg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Chicken IgY H&L Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Chicken IgY H&L Antibody (Ab170784) - SDS-PAGE
3 μg/lane of Goat Anti-Chicken IgY H&L Antibody (Ab170784) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining. Showing the band at 26.1 kDa (Light Chain) & 53.1 kDa (Heavy Chain), under reducing conditions; 162.9 kDa, under non-reducing conditions.
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