| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab170451-1mg | 1mg |
现货  |
| |
| Ab170451-5mg | 5mg |
现货 |
| |
| Ab170451-10mg | 10mg |
期货 |
|
| 产品名称 | Rabbit anti-mouse IgG antibody |
|---|---|
| 规格或纯度 | ≥95％ |
| 应用 | WB,CLIA,IHC,LF,EIA |
| 纯化方法 | antigen affinity chromatography |
|---|---|
| 物理外观 | liquid |
| 储存缓冲液 | 10mM phosphate buffered saline (PBS), pH7.4 |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
Rabbit anti-mouse IgG antibody (Ab170451) - ELISA
Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The plate was coated with different amounts of mouse IgG (Ab170221) (3-fold started from 100 ng/mL). Rabbit anti-mouse IgG antibody was then added at a series of different dilution ratios. Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.
Rabbit anti-mouse IgG antibody (Ab170451) - ELISA
Cross-reactivity of Rabbit anti-mouse IgG antibody (Ab170451) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 μg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Rabbit anti-mouse IgG antibody was then added at different dilution ratio followed by incubation for 1h. Donkey Anti-Rabbit IgG H&L (Biotin) (Ab136895) was used at 1/10000 dilution as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/10000 dilution for this ELISA.
For the batch tested, Rabbit anti-mouse IgG antibody (Ab170451) showed a cross-reactivity with Human IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/64000.
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