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Goat Anti-Human IgG Antibody

Goat Anti-Human IgG Antibody

规格或纯度: ≥ 95%

亚型: Human IgG1

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab170447-1mg	1mg	现货
Ab170447-5mg	5mg	现货
Ab170447-10mg	10mg	现货

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基本信息

产品名称	Goat Anti-Human IgG Antibody
规格或纯度	≥ 95%
应用	ELISA,CLIA,LFAs,WB and other immunoassay methods

产品属性

亚型	Human IgG1
纯化方法	ntigen affinity chromatography
物理外观	liquid
储存缓冲液	10mM phosphate buffered saline (PBS), pH7.4
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输

图片

ELISA: Goat Anti-Human IgG Antibody (Ab170447)

Goat Anti-Human IgG Antibody (Ab170447) - ELISA
Goat Anti-Human IgG Antibody (Ab170447) was tested by ELISA. The plate was coated with different amounts of human IgG (Ab170213) (3-fold started from 1000 ng/mL. Goat Anti-Human IgG Antibody (Ab170447) was then added at a series of different dilution ratios. Rabbit-Anti-Goat IgG H&L HRP (Ab141534) was used at 1/10000 dilution as the secondary antibody.

ELISA: Goat Anti-Human IgG Antibody (Ab170447)

Goat Anti-Human IgG Antibody (Ab170447) - ELISA
Cross-reactivity of Goat Anti-Human IgG Antibody (Ab170447) was tested by ELISA. The wells were coated with the indicated IgG standards at 1 μg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Human IgG Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit-Anti-Goat IgG H&L HRP (Ab141534) was used at 1/10000 dilution as the secondary antibody.
For the batch tested, Goat Anti-Human IgG Antibody (Ab170447) showed a cross-reactivity with rabbit IgG when the dilution ratio of Rabbit anti-mouse IgG antibody is less than 1/8000.