| 产品名称 | Goat Anti-Rabbit IgG H&L (HRP) |
|---|---|
| 规格或纯度 | Secondary Antibody; Goat Anti-Rabbit IgG H&L (HRP); WB, ELISA, IHC |
| 特异性 | Rabbit IgG |
| 交叉反应 | No cross reaction was detected with human IgG, mouse IgG, horse IGG, pig IgG and chicken IGY. |
| 免疫原 | Rabbit IgG |
| 阳性对照 | ELISA: Rabbit IgG |
| 克隆 | Polyclonal |
|---|---|
| Format | Whole IgG |
| 物理外观 | Liquid |
| 储存缓冲液 | Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300. |
| 防腐剂 | 0.05% Proclin300 |
| 浓度 | 1.0 mg/mL |
| 储存温度 | 避光,-20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light. |
| Application | Notes |
|---|---|
| WB | 1/10000-1/200000 |
| ELISA | 1/5000-1/100000 |
Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) - Western Blot
All lanes: Recombinant gamma Tubulin Antibody (Ab104713) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 48 kDa
Exposure time: 1.2 s
Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) - ELISA
Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) was tested by direct ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215). A serial dilution of Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) was used as the secondary antibody.
Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) - ELISA
Cross-reactivity of Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) was tested by direct ELISA . The wells were coated with the indicated IgG at 0.5 μg/mL (100 μL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution was used as the primary antibody.
首页
400-620-6333
危险品化学品经营许可证（带存储）