| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
|
Ab120681-10μl (试用装) 申请此免费试用装（?） 作为尊贵的客户，您每年可免费申请三次您选择的不同货号试用装产品，尽享探索与享受！
|
10μl |
期货  |
| |
|
Ab120681-50μl |
50μL |
现货 |
| |
|
Ab120681-100μl |
100μL |
现货 |
| |
|
Ab120681-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant PCNA Antibody |
|---|---|
| 规格或纯度 | Recombinant (RR704); Rabbit anti Human PCNA Antibody; WB, Flow, IP; Unconjugated |
| 特异性 | PCNA |
| 反应种属 | Human |
| 预测反应种属 | Mouse,Rat |
| 交叉反应 | Cross-reactivity with other species has not been measured. |
| 免疫原 | A synthetic peptide corresponding to residues of human PCNA was used as an immunogen (AA 100-200). |
| 阳性对照 | WB: A431, HEK293, MCF7, HeLa, Hep G2, NIH/3T3, C6, K562, Jurkat and HUVEC cell lysates. Flow: HeLa cells. IP: HeLa whole cell lysate. IF/ICC: HeLa cells. |
| 偶联标记 | Unconjugated |
| 克隆 | Recombinant |
|---|---|
| 克隆号 | RR704 |
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3 |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.5 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| Flow | 1/800 - 1/2000 |
| WB |
1/1,000-1/20,000
|
| IP |
1/50
|
| WB |
1/1000 - 1/2000
|
| IF/ICC | 1/1000 |
Recombinant PCNA Antibody (Ab120681) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 14.1 s
Recombinant PCNA Antibody (Ab120681) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant PCNA Antibody (Ab120681) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant PCNA Antibody (Ab120681) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant PCNA Antibody (Ab120681) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant PCNA Antibody (Ab120681) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor? 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant PCNA Antibody (Ab120681) - IP
PCNA was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with Recombinant PCNA Antibody (Ab120681) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant PCNA Antibody (Ab120681) IP in HeLa whole cell lysate
Lane 2: PBS instead of Recombinant PCNA Antibody (Ab120681) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).
首页
400-620-6333
危险品化学品经营许可证（带存储）