| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
|
Ab101839-10μl (试用装) 申请此免费试用装（?） 作为尊贵的客户，您每年可免费申请三次您选择的不同货号试用装产品，尽享探索与享受！
|
10μl |
期货  |
| |
|
Ab101839-50μl |
50μL |
现货 |
| |
|
Ab101839-100μl |
100μL |
现货 |
| |
|
Ab101839-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant EpCAM Antibody |
|---|---|
| 规格或纯度 | Recombinant (RR694); Rabbit anti Human EpCAM Antibody; WB, IHC, Flow, IP; Unconjugated |
| 特异性 | EpCAM |
| 反应种属 | Human |
| 免疫原 | A synthetic peptide corresponding to residues of human EPCAM. (AA 1-100) |
| 阳性对照 | WB: Hep G2, HEK293 and A431 cell lysates. IHC: Human Uterus tissue. Flow: HCT-116 cells. IP: HT-29 whole cell lysate. |
| 偶联标记 | Unconjugated |
| 克隆 | Recombinant |
|---|---|
| 克隆号 | RR694 |
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 59% 10 mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3. |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.5 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| WB |
1/2000 - 1/5000
|
| Flow | 1/800 - 1/2000 |
| IP |
1/50
|
| IHC |
1/1600-1/3200
|
Recombinant EpCAM Antibody (Ab101839) - Western Blot
All lanes: Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 39 kDa
Exposure time: 5.0 s
Recombinant EpCAM Antibody (Ab101839) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling EpCAM with Recombinant EpCAM Antibody (Ab101839) at 1/3200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant EpCAM Antibody (Ab101839) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained with Recombinant EpCAM Antibody (Ab101839) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant EpCAM Antibody (Ab101839) ( 1/800 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor? 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant EpCAM Antibody (Ab101839) - IP
EpCAM was immunoprecipitated from 0.4 mg of HT-29 whole cell lysate with Recombinant EpCAM Antibody (Ab101839) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant EpCAM Antibody (Ab101839) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant EpCAM Antibody (Ab101839) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 120 s
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