| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab094381-50μl | 50μL |
现货  |
| |
| Ab094381-100μl | 100μL |
现货 |
| |
| Ab094381-1ml | 1ml |
期货 |
|
| 产品名称 | CD276 Antibody |
|---|---|
| 规格或纯度 | pAb; Rabbit anti Human CD276 Antibody; WB, IHC, Flow; Unconjugated |
| 特异性 | CD276 |
| 反应种属 | Human |
| 免疫原 | Recombinant CD276 expressed in E.coli (AA 47-219) |
| 阳性对照 | WB: HeLa, LNCAP, HEK293 and MCF7 cell lysates. IHC: Human Liver cancer tissue, Human Kidney tissue. Flow: CHO-K1 transfected with Human CD276. |
| 克隆 | Polyclonal |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Antigen affinity purified and Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10 mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4 |
| 防腐剂 | 0.05% Proclin 300 |
| 浓度 | Lot by Lot |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| IHC | 5-30 μg/mL |
| WB | 0.01-3 μg/mL |
CD276 Antibody (Ab094381) - Western Blot
All lanes: CD276 Antibody (Ab094381) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 92 kDa
Exposure time: 71.7 s
CD276 Antibody (Ab094381) - Flow Cytometry
CHO-K1 transfected with human CD276 (Stable transfection) was stained with CD276 Antibody (Ab094381) (filled red histogram) or Isotype control antibody (filled blue histogram), followed by Cy5-conjugated Anti-rabbit IgG Secondary Antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
CD276 Antibody (Ab094381) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 μg/mL CD276 Antibody (Ab094381)
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
CD276 Antibody (Ab094381) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 μg/mL CD276 Antibody (Ab094381)
Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Secondary antibody only control: Used PBS instead of primary antibody Second Ab: 2 μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
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