| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
|
Ab091197-10μl (试用装) 申请此免费试用装（?） 作为尊贵的客户，您每年可免费申请三次您选择的不同货号试用装产品，尽享探索与享受！
|
10μl |
期货  |
| |
|
Ab091197-50μl |
50μL |
现货 |
| |
|
Ab091197-100μl |
100μL |
现货 |
| |
|
Ab091197-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant beta Tubulin Antibody |
|---|---|
| 规格或纯度 | Recombinant (R09-3F4); Rabbit anti Human Beta Tubulin Antibody; WB, IHC, ICC, IF; Unconjugated |
| 特异性 | TUBB |
| 反应种属 | Human,Mouse?,Rat? |
| 预测反应种属 | African green monkey |
| 交叉反应 | Cross-reactivity with other species has not been measured. |
| 免疫原 | A synthetic peptide derived from human beta Tubulin (AA 324-337). |
| 阳性对照 | WB: K562, C6, NIH3T3, HeLa, A431, Jurkat, HEK293 and MCF7 cell lysates. IHC: Human brain tissues. IF/ICC: HeLa cells. |
| 偶联标记 | Unconjugated |
| 克隆 | Recombinant |
|---|---|
| 克隆号 | R09-3F4 |
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Antigen affinity purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4 |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.2 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/200 |
| IF/ICC | 1/100 - 1/200 |
Recombinant beta Tubulin Antibody (Ab091197) - Western Blot
All lanes: Recombinant beta Tubulin Antibody (Ab091197) at 1/5000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 15.0 s
Recombinant beta Tubulin Antibody (Ab091197) - ICC/IF
Immunocytochemistry analysis of beta Tubulin(green) in HeLa cells using Recombinant beta Tubulin Antibody (Ab091197) and cells were counterstained with DAPI (blue).
Recombinant beta Tubulin Antibody (Ab091197) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using Recombinant beta Tubulin Antibody (Ab091197) at 1/50 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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