| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab087287-100μl | 100μL |
现货  |
| |
| Ab087287-1ml | 1ml |
期货 |
| |
| Ab087287-50μl | 50μL |
现货 |
|
| 产品名称 | NOTCH1 Antibody |
|---|---|
| 规格或纯度 | pAb; Rabbit anti Human NOTCH1 Antibody; WB, IHC, ICC, IF; Unconjugated |
| 特异性 | NOTCH1 |
| 反应种属 | Human,Mouse?,Rat? |
| 免疫原 | A synthetic peptide derived from human Notch1 (AA 2505-2519). |
| 阳性对照 | WB: MCF-7, Jurkat, HEK293 cell lysates; IHC: Human liver; IF: HeLa. |
| 克隆 | Polyclonal |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 150mM NaCl, 50% Glycerol, 0.02% Sodium azide, pH 7.4. |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | 1.5 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
| IF/ICC | 1/50 – 1/200 |
NOTCH1 Antibody (Ab087287) - Western Blot
All lanes: NOTCH1 Antibody (Ab087287) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 120 kDa
Observed band size: 112 kDa
Exposure time: 3.0 s
NOTCH1 Antibody (Ab087287) - IHC
Immunohistochemical analysis of paraffin-embedded Human liver using NOTCH1 Antibody (Ab087287). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
NOTCH1 Antibody (Ab087287) - ICC/IF
Immunofluorescent analysis of NOTCH1 in HeLa cells using NOTCH1 Antibody (Ab087287).
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