| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab087103-50μl | 50μL |
现货  |
| |
| Ab087103-100μl | 100μL |
现货 |
| |
| Ab087103-1ml | 1ml |
期货 |
|
| 产品名称 | ACAT1 Antibody |
|---|---|
| 规格或纯度 | pAb; Rabbit anti Human ACAT1 Antibody; WB, IHC, IF, ICC; Unconjugated |
| 特异性 | ACAT1 |
| 反应种属 | Human,Mouse? |
| 免疫原 | Recombinant ACAT1 expressed in E.coli (AA 111-427) |
| 阳性对照 | WB: A549, HEK293, A431, MOLT-4, Jurkat, Hep G2 and NIH/3T3 cell lysates. IHC: Human Kidney Tissue, Human Lymph node Tissue. ICC/IF: Hep G2 cells. |
| 克隆 | Polyclonal |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Antigen-specific affinity chromatography followed by Protein A affinity chromatography. |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4. |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | Lot by Lot |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| IHC | 5-20 μg/mL |
| ICC/IF |
5-20 μg/mL
|
| WB | 0.5-2 μg/mL |
ACAT1 Antibody (Ab087103) - Western Blot
All lanes: ACAT1 Antibody (Ab087103) at 1/1000 dilution
Samples: Lysates at 20 μg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 40 kDa
Exposure time: 19.8 s
ACAT1 Antibody (Ab087103) - IF
FITC staining on IF
Samples: Human Hep G2 cells
Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103)
Second Ab: 1.5μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ACAT1 Antibody (Ab087103) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103)
Second Ab: 2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
ACAT1 Antibody (Ab087103) - IHC
DAB staining on IHC
Samples: Human Lymph node Tissue
Primary Ab: 20μg/mL ACAT1 Antibody (Ab087103)
Second Ab: 2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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