7-氨基放线菌素D(7-AAD)
- CAS编号: 7240-37-1
- 分子式: C62H87N13O16
- 分子量: 1270.43
- MDL号: MFCD00077295
- PubChem编号: 14924508
库存信息
库存信息
库存信息
货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
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A131295-1mg | 1mg | 现货 | | |
A131295-5mg | 5mg | 现货 | | |
A131295-25mg | 25mg | 现货 | |
货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
---|---|---|---|---|
A131295-1mg | 1mg | 现货 | | |
A131295-5mg | 5mg | 现货 | | |
A131295-25mg | 25mg | 现货 | |
规格或纯度 | ≥97.0% (HPLC) |
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英文名称 | 7-Aminoactinomycin D |
英文别名 | 2,7-Diamino-N,N'-bis[(6S,9R,10S,13R,18aS)-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1,4,7,10,13]oxatetraazacyclohexadecin-10-yl]-4,6-dimethyl-3-oxo-3H-phenox azine-1,9-dicarboxamide;NSC 239759;7-AAD |
生化机理 | 用于细胞凋亡和流式细胞术研究的荧光DNA染料。放线菌素D的鸟嘌呤特异性合成荧光类似物。通过结合到GC区域插入DNA。区分活细胞以及流式细胞仪中的早期和晚期凋亡。 |
储存温度 | 避光,-20°C储存 |
运输条件 | 超低温冰袋运输 |
备注 | 如果有可能,您尽量在使用的当天配置溶液,并在当天使用完它。但是,如果您需要预先配制储备溶液,我们建议您将溶液等份保存在-20°C的密封小瓶中。通常,它们最多可以使用一个月。在使用前和打开样品瓶之前,我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度,用法和处理的建议吗?请访问我们的常见问题(FAQ)页面以获取更多详细信息。 |
产品介绍 |
7-Aminoactinomycin D ,an analog of the antibiotic actinomycin D ,is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA PolymeraseA fluorescent DNA dye for apoptosis and flow cytomtery studies. 7-Aminoactinomycin D ,an analog of the antibiotic actinomycin D ,is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase |
IUPAC Name | 2,7-diamino-4,6-dimethyl-3-oxo-1-N,9-N-bis[(3R,6S,7R,10S,16S)-7,11,14-trimethyl-2,5,9,12,15-pentaoxo-3,10-di(propan-2-yl)-8-oxa-1,4,11,14-tetrazabicyclo[14.3.0]nonadecan-6-yl]phenoxazine-1,9-dicarboxamide |
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INCHI | InChI=1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)/t32-,33-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1 |
InChi Key | YXHLJMWYDTXDHS-IRFLANFNSA-N |
Canonical SMILES | CC1C(C(=O)NC(C(=O)N2CCCC2C(=O)N(CC(=O)N(C(C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=CC(=C(C4=C3N=C5C(=C(C(=O)C(=C5O4)C)N)C(=O)NC6C(OC(=O)C(N(C(=O)CN(C(=O)C7CCCN7C(=O)C(NC6=O)C(C)C)C)C)C(C)C)C)C)N |
分子式 |
C62H87N13O16 |
PubChem CID | 14924508 |
分子量 | 1270.43 |
溶解性 | Soluble in DMSO (20 mg/ml), ethanol, chloroform (1 mg/ml), and dichloromethane (20 mg/ml). |
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敏感性 | 对光和湿度敏感 |
熔点 | 252-253°C |
象形图 |
Toxic Health Hazard Harmful |
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信号词 | Danger |
危险声明 |
H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation H300: Fatal if swallowed H310: Fatal in contact with skin H330: Fatal if inhaled H360: May damage fertility or the unborn child H350: May cause cancer |
预防措施声明 | P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P260,P284,P271,P270,P304+P340,P403+P233,P362+P364,P330,P262,P320,P361+P364,P203,P264+P265,P301+P316,P318,P337+P317,P332+P317,P316,P319 |
WGK Germany | 3 |
RTECS | AU1579000 |
个人防护装备 | Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
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