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低电渗琼脂糖

规格或纯度: low EEO
有货

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货号 (SKU) 包装规格 是否现货 价格 数量
A118880-1g 1g 现货 Stock Image
A118880-5g 5g 现货 Stock Image
A118880-25g 25g 现货 Stock Image
A118880-100g 100g 现货 Stock Image
A118880-500g 500g 现货 Stock Image

基本描述

规格或纯度 low EEO
英文名称 Agarose
别名 琼脂糖凝胶
英文别名 LE Agarose
生化机理 由于其生物相容性、机械强度以及在低温下的胶凝能力等特性,琼脂糖可用作生物墨水。
储存温度 室温,干燥
运输条件 常规运输
产品介绍

有吸湿性。溶于热水,不溶于冷水、有机溶剂。适当浓度的水溶液,遇冷凝结成胶状。在低pH时,凝胶强度下降,甚至不能成胶状,冰冻的凝胶解冻后凝胶形状会破坏,但加热溶解放冷后可重新形成原来形状。 琼脂属于半乳聚糖多糖,其构成了属于红藻纲的红色海藻/海藻中的细胞内基质。琼脂天然具有轻微的硫酸化,是一种多糖的复杂混合物。琼脂糖是琼脂的一种中性聚合物成分。对琼脂糖加热可生成溶液的形式,而它在冷却时则会凝固成凝胶形式。凝胶形成的发生是由于来自分子链的双螺旋形成,而它可形成水固定的网络。脱氧核糖核酸(DNA),脂蛋白和免疫电泳用。生化研究免疫扩散等的底物。生物学、免疫学、生物化学和微生物学研究。临床医学上用于乙型肝炎抗原(HAA)测定。血电泳分析。甲胎球蛋白测定。肝炎、肝癌和心血管等疾病的诊断。 常规琼脂糖是通过凝胶电泳或印迹(Northern 或 Southern)进行日常核酸分析的理想选择,还适用于蛋白质应用,例如 Ouchterlony 免疫扩散和放射状免疫扩散 (RID)。含少量溴化乙锭和 SYBR Green 背景染料。


产品类型

Type I;technique(s):electrophoresis: suitable;转变温度:gel point 36 °C ±1.5 °C (1.5% gel);杂质:≤10% water; EEO:0.09-0.13;凝胶强度;≥1200 g/cm2 (1% gel);痕量阴离子:sulfate (SO42-): ≤0.15%;


产品说明
琼脂糖是一种琼脂二糖(L-和D-半乳糖)亚基的聚合物。它是从石花菜属和江篱藻属海藻中提取获得的。琼脂糖能够形成非共价捆绑网状结构。它可用于分离核酸。浓度和胶孔径是负相关。琼脂糖分离范围广,简便,准备方便,不需要催化剂。

产品用途
琼脂糖已用于:嵌入荧光纳米球;构建类似于心肌组织的组织样体模;水凝胶制备;

分析说明
以下是与我们的琼脂糖相关的性质列表:
硫酸盐含量 - 用作纯度指标,因为硫酸盐是其中存在的主要离子基团。
凝胶强度 - 破裂凝胶所需施加的力。
胶凝点 - 琼脂糖水溶液在冷却时的胶凝温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说,它们的凝胶点与它们的熔点温度不同。
电内渗(EEO)- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动,但可解离的抗衡阳离子可以向基质中的阴极迁移,从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的,因此EEO会因为内部的对流而破坏分离。

Agar belongs to the galactan polysaccharides, which make up the intracellular matrix in red seaweeds/marine algae belonging to class Rhodophyta. Agar is naturally slightly sulfated, and is a complex mixture of polysaccharides. Agarose is a neutral polymer component of agar. Heating of agarose gives rise to solution form which on cooling sets into gel form. Gel formation occurs due to double helical formation from molecular chains, which forms a water-immobilizing network.

名称和标识符

EC号 232-731-8
IUPAC Name (2S,3R,4S,5R,6R)-2-[[(1S,3S,4S,5S,8R)-3-[(2S,3R,4S,5S,6R)-2-[[(1S,3R,4S,5S,8R)-3,4-dihydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy]-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-4-hydroxy-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
INCHI InChI=1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChi Key MJQHZNBUODTQTK-WKGBVCLCSA-N
Canonical SMILES C1C2C(C(O1)C(C(O2)O)O)OC3C(C(C(C(O3)CO)O)OC4C(C5C(C(O4)CO5)OC6C(C(C(C(O6)CO)O)O)O)O)O
分子式

C24H38O19

关联CAS 39346-81-1
PubChem CID 11966311
分子量 630.5

化学和物理性质

溶解性 Soluble in water.
敏感性 对湿度敏感
熔点 62-68℃

参考文献

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