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基本描述
| 规格或纯度 | 足以进行100次荧光检测 |
|---|---|
| 英文名称 | Thrombin Activity Fluorometric Assay Kit |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
描述 凝血酶（因子 IIa，EC 3.4.21.5）是一种丝氨酸蛋白酶，是凝血级联中的重要凝血因子，其参与可溶性纤维蛋白原转化为不溶性活性纤维蛋白链的过程。在该途径中，凝血酶原被蛋白水解转化为活性凝血酶。 应用 适用性 适用于测定纯凝血酶的活性和检测血浆中凝血酶的活性。 原理 凝血酶活性荧光检测试剂盒利用凝血酶的能力，蛋白水解地切割合成底物，并释放荧光团7-氨基-4-甲基香豆素（AMC），其可通过荧光很容易地定量。该检测试剂盒简单、快速，可检测样品中低至 1ng的凝血酶活性。 制备说明 外形 Description Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin.Suitability: Suitable for determining the activity of pure Thrombin and for detecting the activity of Thrombin in plasma.Principle: Thrombin activity fluorometric assay kit utilizes the ability of thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, 7-amino-4-methylcoumarin (AMC), which can be easily quantified by fluorescence. This assay kit is simple, rapid and can detect thrombin activity as low as 1ng in samples.} |
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