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重组胰蛋白酶细胞消化液
规格或纯度:
pH 7.2～7.4
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基本描述
| 规格或纯度 | pH 7.2～7.4 |
|---|---|
| 英文名称 | Trypsin solution |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
组成：重组胰蛋白酶冻干粉，无Ca2+ ，Mg2+ ，无EDTA的平衡液，pH7.2～7.4，无菌过滤。 重组生产，保证了无动物源性病毒污染。使用安全。冻干剂型，即配即用，固体包装，降低了污染风险。基因工程重组生产，HPLC纯化，不含杂酶、杂蛋白及脂类、DNA等。细胞消化能力强：室温消化，高纯度，避免了杂质对细胞生长的影响。在缓冲液中不含有EDTA,但是保持高的细胞消化能力，排除了细胞流式实验中EDTA的影响。 Recombinant trypsin solution, is an animalcomponent free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.The trypsin solution, readyto-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis. UNIT DEFINITION:One BAEE unit of trypsin activity will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃ (1cm light path) |
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