| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| T109028-500ml | 500ml |
现货  |
| |
| T109028-12×500ml | 12×500ml |
现货 |
|
| 规格或纯度 | 电泳级 |
|---|---|
| 英文名称 | Triton? X-100 |
| 别名 | 4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, 叔辛基苯氧基聚乙烯乙氧基乙醇, 聚乙二醇叔辛基苯基醚 |
| 英文别名 | t-Octylphenoxypolyethoxyethanol Polyethylene glycol tert-octylphenyl ether Polyoxyethylene(10) isooctylphenyl ether Triton X-100 |
| 生化机理 | 广泛使用的非离子表面活性剂，用于在温和的非变性条件下进行膜恢复。 |
| 储存温度 | 室温 |
| 运输条件 | 常规运输 |
| 产品介绍 |
General description: Triton? X-100是一种常用的非离子型的表面活性剂和乳化剂，经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的去污剂，并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。它还可通透活细胞膜用于转染。 Application: Triton? X-100已用于： ? 免疫组化用于平面视网膜的染色 ? 与冰冷的PBS（磷酸盐缓冲液）一起悬浮细胞用于细胞DNA分析和Annexin V检测 ? 在免疫荧光显微研究中对细胞进行通透化处理 ? 在LDH检测中作为阳性对照用于测定细胞膜的完整性 ? 采用改良的Belfrage和Vaughan放射性酶法过程测定肝素后血浆中的脂肪酶活性 ? 外层膜蛋白提取物的制备 ? 作为提取缓冲液中一种成分与tris-HCl、NaCl、CaCl2、ZnCl2、Brij 35一起用于小鼠肺细胞的匀浆 ? 免疫荧光标记的组织切片处理 General description Triton? X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection Application Triton? X-100 has been used: ? In immunohistochemistry for staining the Flat-mount retinas ? Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay ? To permeabilise cells during Immunofluorescent microscopic studies ? As a positive control in LDH assay to determine the cell membrane integrity ? For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure ? For the preparation of outer membrane protein exctract ? As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells ? In the treatment of tissue sections for Immunofluorescence labeling |
| EC号 | 232-658-1 |
|---|---|
| IUPAC Name | 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol |
| INCHI | InChI=1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3 |
| InChi Key | JYCQQPHGFMYQCF-UHFFFAOYSA-N |
| Canonical SMILES | CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO |
| 分子式 |
C8H17C6H4(OCH2CH2)nOH(n≈10) |
| UN Number | 3082 |
| PubChem CID | 5590 |
| Beilstein号 | 2315025 |
| 密度 | 1.01 |
|---|---|
| 沸点 | 270°C |
| 熔点 | 6°C |
| 折光率 | 1.491 |
| 闪点(℉) | 251 °C |
| 闪点(℃) | 251°C |
| 象形图 |
Environmental Hazard 
Corrosive 
Harmful |
|---|---|
| 信号词 | Danger |
| 危险声明 |
H315: Causes skin irritation H319: Causes serious eye irritation H411: Toxic to aquatic life with long lasting effects H412: Harmful to aquatic life with long lasting effects H302: Harmful if swallowed H318: Causes serious eye damage H400: Very toxic to aquatic life H410: Very toxic to aquatic life with long lasting effects |
| 预防措施声明 | P305+P351+P338,P273,P280,P302+P352,P321,P501,P264,P270,P362+P364,P391,P330,P264+P265,P301+P317,P305+P354+P338,P317,P337+P317,P332+P317 |
| WGK Germany | 1 |
| RTECS | MD0907700 |
| Merck Index | 6761 |
| 个人防护装备 | Eyeshields,Faceshields,full-face respirator (US),Gloves,multi-purpose combination respirator cartridge (US),type ABEK (EN14387) respirator filter |
尊敬的客户您好，
? ? ? ?您在我司提交的【T109028曲拉通X-100(Triton? X-100)】咨询问题,给您的回复如下：
您好，该产品是纯品，纯度未检测，但生产工艺是一个成熟的实验体系，属于高纯试剂，临界胶束浓度大概为0.2X10-3 mol/L。
? ? ? ? ?祝您工作愉快！欢迎您再次咨询。
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 上海阿拉丁生化科技股份有限公司
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 2022年7月5日
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