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曲拉通X-100(Triton? X-100)

规格或纯度: 分子生物学级
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聚合物/高分子试剂 醇
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基本描述

规格或纯度 分子生物学级
英文名称 Triton? X-100
别名 4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, 叔辛基苯氧基聚乙烯乙氧基乙醇, 聚乙二醇叔辛基苯基醚
英文别名 t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
生化机理 广泛使用的非离子表面活性剂，用于在温和的非变性条件下进行膜恢复。
储存温度 室温
运输条件 常规运输
产品介绍

General description:

Triton? X-100是一种常用的非离子型的表面活性剂和乳化剂，经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的去污剂，并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。它还可通透活细胞膜用于转染。


Application:

Triton? X-100已用于：

? 免疫组化用于平面视网膜的染色

? 与冰冷的PBS（磷酸盐缓冲液）一起悬浮细胞用于细胞DNA分析和Annexin V检测

? 在免疫荧光显微研究中对细胞进行通透化处理

? 在LDH检测中作为阳性对照用于测定细胞膜的完整性

? 采用改良的Belfrage和Vaughan放射性酶法过程测定肝素后血浆中的脂肪酶活性

? 外层膜蛋白提取物的制备

? 作为提取缓冲液中一种成分与tris-HCl、NaCl、CaCl2、ZnCl2、Brij 35一起用于小鼠肺细胞的匀浆

? 免疫荧光标记的组织切片处理

General description

Triton? X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection


Application

Triton? X-100 has been used:

? In immunohistochemistry for staining the Flat-mount retinas

? Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay

? To permeabilise cells during Immunofluorescent microscopic studies

? As a positive control in LDH assay to determine the cell membrane integrity

? For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure

? For the preparation of outer membrane protein exctract

? As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells

? In the treatment of tissue sections for Immunofluorescence labeling

名称和标识符

EC号 232-658-1
IUPAC Name 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol
INCHI InChI=1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3
InChi Key JYCQQPHGFMYQCF-UHFFFAOYSA-N
Canonical SMILES CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO
分子式

C8H17C6H4(OCH2CH2)nOH(n≈10)
UN Number 3082
PubChem CID 5590
Beilstein号 2315025

化学和物理性质

密度 1.01
沸点 270°C
熔点 6°C
折光率 1.491
闪点(℉) 251 °C
闪点(℃) 251°C

安全信息

象形图
ghs09

Environmental Hazard

ghs05

Corrosive

ghs07

Harmful
信号词 Danger
危险声明 H315: Causes skin irritation
H319: Causes serious eye irritation
H411: Toxic to aquatic life with long lasting effects
H412: Harmful to aquatic life with long lasting effects
H302: Harmful if swallowed
H318: Causes serious eye damage
H400: Very toxic to aquatic life
H410: Very toxic to aquatic life with long lasting effects
预防措施声明 P305+P351+P338,P273,P280,P302+P352,P321,P501,P264,P270,P362+P364,P391,P330,P264+P265,P301+P317,P305+P354+P338,P317,P337+P317,P332+P317
WGK Germany 1
RTECS MD0907700
Merck Index 6761
个人防护装备 Eyeshields,Faceshields,full-face respirator (US),Gloves,multi-purpose combination respirator cartridge (US),type ABEK (EN14387) respirator filter

参考文献

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