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无血清（Serum Free）细胞冻存液
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基本描述
| 英文名称 | Serum free cell cryopreservation solution |
|---|---|
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
产品介绍： 无血清（Serum Free）细胞冻存液为即用型产品，不含血清和动物来源性蛋白。因此，不仅降低了各类细菌、病毒、支原体等污染风险，保证冻存细胞的安全，而且减少外源性蛋白对细胞正常生长和分化的影响。此外，无需程序降温，可长期-80℃保存细胞，适用于绝大多数哺乳动物细胞的冻存。 注意事项： 1. 使用前确保细胞冻存液彻底融化，并轻轻混匀。融化后的细胞冻存液置于4oC保存，三个月内有效。 2. 本产品含有酚红指示剂，长时间敞口会导致PH变化，并出现沉淀。故使用过程中请尽量缩短敞口时间，使用完毕后盖紧盖子保存，如有必要可适当分装使用。若使用一段时间后出现少量沉淀，经测试对细胞冻存无影响，去除沉淀可采取离心取上清、0.22 μM孔径过滤器过滤等方法去除。 3. 细胞冻存前请确保其状态良好，活细胞比例宜大于90%。 4. 本产品含DMSO，部分对DMSO敏感的细胞，建议进行预实验。 5. 对于某些珍贵的细胞，建议同时使用含FBS的常规冻存液进行对比测试，确认性能后再进行正式冻存。 6. 冻存细胞分装后应尽快移入-80oC超低温冰箱内，减少在外存放时间。 7. 为了您的安全和健康，请穿实验服并戴一次性手套操作。 应用范围： 细胞冻存 Product introduction： Uelandy serum free cell crYOpreservation solution is a ready to use product without serum and animal derived protein. Therefore, it not only reduces the pollution risk of all kinds of bacteria, viruses, mycoplasma, etc., and ensures the safety of frozen cells, but also reduces the impact of exogenous proteins on the normal growth and differentiation of cells. In addition, without the need for programmed cooling, cells can be stored at -80 ℃ for a long time, which is suitable for the crYOpreservation of most mammalian cells. Matters needing attention： 1. before use, ensure that the cell cryopreservation solution is completely melted and gently mixed. The thawed cell cryopreservation solution is stored at 4 o C and is effective within three months. 2. this product contains phenol red indicator. Long time exposure will lead to pH change and precipitation. Therefore, please try to shorten the exposure time during use. After use, close the cover tightly and store it. If necessary, it can be properly subpackaged for use. If a small amount of precipitate appears after using for a period of time, it has no effect on cell cryopreservation after testing. To remove the precipitate, centrifuge the supernatant, 0.22 μ M pore size filter filtration and other methods. 3. please ensure that the cells are in good condition before freezing, and the proportion of viable cells should be greater than 90%. 4. this product contains DMSO, and some cells are sensitive to DMSO. It is recommended to conduct pre experiment. 5. for some precious cells, it is recommended to use the conventional freezing solution containing FBS for comparison test at the same time, and then conduct formal freezing after confirming the performance. 6. after subpackaging, the frozen cells should be moved into the -80 o C ultra-low temperature refrigerator as soon as possible to reduce the storage time outside. 7. for your safety and health, please wear experimental clothes and disposable gloves. Scope of application： Cell cryopreservation |
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