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Super Green II RNA凝胶染料
规格或纯度:
10,000× in DMSO
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基本描述
| 规格或纯度 | 10,000× in DMSO | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 英文名称 | Super Green II RNA gel stain | ||||||||
| 储存温度 | 2-8°C储存,避光,干燥 | ||||||||
| 运输条件 | 冰袋运输 | ||||||||
| 产品介绍 |
Super Green效果与Sybr Green一样，本品用DMSO溶解，因DMSO的熔点是18.5℃，使用前请放置到室温充分溶解。 Super Green II核酸染料特点 无毒性：属花箐类染料，容易生物降解，无致癌毒性。 灵敏度高：至少可检出20pg ssDNA或RNA，高于EB染色法25～100倍。 信噪比高：样品荧光信号强，背景信号低。 操作简单：与EB?一样，在预制胶和电泳过程中染料不降解；而电泳后染色过程也只需30?分钟且无需脱色或冲洗，即可直接用紫外凝胶透射仪或可见光透射仪观察。 适用范围广：可选择电泳前染色（胶染法）或电泳后染色（泡染法）；适用于琼脂糖凝胶电泳、聚丙烯酰胺凝胶电泳、脉冲电场凝胶电泳和毛细管电泳等；可用于ssDNA?或RNA?染色。 使用方便：对分子生物学中常用的酶（如：Taq?酶、反转录酶、内切酶、T4连接酶等）没有抑制作用。?? 经济：价格比银染便宜。 Super Green II使用方法简介 1．胶染法（用法同EB）（推荐方法） （1） 制胶时加入Super Green II?核酸染料。冷却胶至50℃左右，每100ml胶中加入3～5μl? Super Green II 10,000×?储液，以此比例类推。 （2） 按照常规方法进行电泳。 注意事项：此方法染色可以准确确定核酸片段分子量，染料用量相对较少。1ml染料大约可以做300块?100ml的胶。 由于Super Green II热稳定性较差，不能在热的胶溶液中直接添加，需要等待溶液冷却至50℃左右才能添加。摇晃，振荡或者翻转以保证染料充分混匀。 2．泡染法 （1）按照常规方法进行电泳。 （2） 用pH 7.0～8.5?的缓冲液（如：TAE，TBE?或TE），按照10000﹕1的比例稀释Super Green II??10,000×?储液，混匀，制成1×?染色液。 （3） 将凝胶小心地放入合适的容器中，如聚丙烯容器中。缓慢加入足量的1×?染色液浸没凝胶。用铝箔等盖住容器使染料避光。室温振荡染色10～30分钟，染色时间因凝胶浓度和厚度而定。聚丙烯酰胺凝胶直接在玻璃平皿上染色，将配好的1×?染色液轻轻地倒在胶板上，让其均匀地覆盖整个胶板，并染色30分钟。玻璃平皿必须预先经过硅烷化溶液处理（避免染料吸附在玻璃表面上）。 注：用泡染法染色时，可以精确确定核酸片段分子量。但染料用量较多。 Super Green II参数
Super Green has the same effect as Sybr Green. This product is dissolved in DMSO. Because the melting point of DMSO is 18.5℃, please put it to room temperature before use to dissolve it. Features of?Super Green II High sensitivity: at least 20pg ssDNA or RNA can be detected, 25-100 times higher than EB staining method High signal-to-noise ratio: strong sample fluorescence signal, low background signal Simple operation: Like EB, the dye does not degrade during the precast gel and electrophoresis; the dyeing process after electrophoresis only takes 30 minutes without decolorization or washing, and can be directly observed with a UV gel transilluminator or a visible light transilluminator Wide application range: can choose staining before electrophoresis (gel staining method) or staining after electrophoresis (bubble staining method); suitable for agarose gel electrophoresis, polyacrylamide gel electrophoresis, pulsed electric field gel electrophoresis and capillary electrophoresis, etc.; available Stain in ssDNA or RNA. Easy to use: It has no inhibitory effect on enzymes commonly used in molecular biology (such as Taq enzyme, reverse transcriptase, endonuclease, T4 ligase, etc.) Economy: The price is cheaper than silver dyeing Introduction to the use of Super Green II 1. Paste dyeing method (the usage is the same as EB) (recommended method) (1) Add Super Green II nucleic acid dye when making gel. Cool the glue to about 50°C, and add 3~5μl Super Green II 10,000× stock solution to every 100ml of glue, and analogy with this ratio (2) Perform electrophoresis in accordance with conventional methods Note: This method of dyeing can accurately determine the molecular weight of nucleic acid fragments, and the amount of dye is relatively small. 1ml of dye can make about 300 pieces of 100ml glue Due to the poor thermal stability of Super Green II, it cannot be added directly to the hot glue solution. It needs to wait for the solution to cool to about 50°C before adding it.Shake, shake or flip to ensure that the dye is fully mixed 2. Bubble dyeing (1) Perform electrophoresis in accordance with conventional methods (2) Dilute the Super Green II 10,000× stock solution with a pH 7.0～8.5 buffer solution (such as TAE, TBE or TE) at a ratio of 10000:1, and mix it to make a 1× staining solution (3) Put the gel carefully into a suitable container, such as a polypropylene container. Slowly add enough 1× staining solution to immerse the gel. Cover the container with aluminum foil to protect the dye from light. Shake and stain at room temperature for 10-30 minutes. The staining time depends on the gel concentration and thickness. Stain the polyacrylamide gel directly on the glass plate. Pour the prepared 1× staining solution gently on the gel plate, let it evenly cover the entire gel plate, and stain for 30 minutes. The glass plates must be pre-treated with a silanization solution (to avoid dyes adsorbing on the glass surface). Note: The molecular weight of nucleic acid fragments can be accurately determined when staining with the bubble staining method. But the amount of dye is higher Super Green II parameters
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化学和物理性质
| 敏感性 | 对光敏感 |
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