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Super Safe 核酸染料
规格或纯度:
10000× in DMSO
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基本描述
| 规格或纯度 | 10000× in DMSO |
|---|---|
| 英文名称 | Super Safe nucleic acid dyes |
| 储存温度 | 2-8°C储存,避光,干燥 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
产品描述 Super Safe是新型无毒核酸染料。这种独特的油性分子是花菁染料，且在凝胶染色浓度下没有诱变性，具有使用安全、检测灵敏等特点，可以作为各种核酸电泳的染色剂，适用于各种片段大小染色。与标准凝胶成像系统和可见光激发的凝胶观察装置完美兼容，适用于紫外凝胶成像系统或蓝色可见光激发的凝胶观察装置。 产品特点 1.安全无毒：尽管可以穿透细胞膜进入细胞内，但是属于花菁染料，在凝胶染色浓度下没有诱变性。 2.灵敏度高：适用于各种大小片段的电泳染色，对核酸迁移的没有任何影响。 3.稳定性高：适用于使用微波或其它加热方法制备琼脂糖凝胶；室温下在酸或碱缓冲液中极其稳定，耐光性强。 4.信噪比高：样品荧光信号强，背景信号低。 5.操作简单：在预制胶和电泳过程中不降解,可直接用可见光凝胶透射仪观察。 6.适用范围广：可选择电泳前染色（胶染法）或电泳后染色（泡染法）；适用琼脂糖凝胶或聚丙烯酰胺凝胶电泳；可用于dsDNA、ssDNA?或RNA?染色。 7.完美兼容：适用于使用254nm?激发的紫外凝胶成像系统或蓝色可见光激发的凝胶观察装置。和SYBR Green?的光谱相似。 储存条件：2-8℃避光干燥可保存12个月。 Ex(nm)?:490 ???????Em(nm)?:520 操作步骤 一、琼脂糖凝胶电泳染色（推荐方法） ? ? 将Super Safe Nucleic Acid Dye加入凝胶中 1．制胶：按常规操作，制备琼脂糖凝胶，加入浓缩的10,000x Super Safe Nucleic Acid Dye，使其在凝胶中的终浓度为1x（例如：制备50ml的凝胶，加入染料5μl），轻轻摇匀，倒胶。 2．按常规方法电泳，观测结果（染料不会影响使DNA迁移！）。 二、泡染法 1．按照常规方法进行电泳。 2．用电泳液将10,000x Super Safe Nucleic Acid Dye储液稀释约3,300倍到纯水中，制成3x染色液。 3．将凝胶小心地放入合适的容器中，如聚丙烯容器中。缓慢加入足量的3x染色液浸没胶。室温振荡染色30min左右。 4 . 在凝胶成像仪内，观测结果 注意事项 1．由于Super Safe?具有良好的热稳定性，可以在热的琼脂糖溶液中直接添加，而不需要等待溶液冷却。摇晃，振荡或者翻转以保证染料充分混匀。也可以选择将Super Safe?储液加到琼脂糖粉末和电泳缓冲液中，然后用微波炉或其他常用方式加热以制备琼脂糖凝胶。Super Safe?兼容所有常用的电泳缓冲溶液。 2．如果条带总是弥散或分离不理想，请使用泡染法染色以确认问题是否与染料有关。如果染色后问题依旧存在，则说明问题与染料无关，请尝试：降低琼脂糖浓度；选用更长的凝胶；延长凝胶时间以保证边缘清晰；改进上样技巧或选择泡染法染色。 3．Super Safe对玻璃器皿和非聚丙烯材料具有一定的亲合力。建议在稀释、贮存、染色等使用过程中用聚丙烯类容器。对于聚丙烯酰胺凝胶请使用泡染法。 Product description Super Safe is a new non-toxic nucleic acid dye. This unique oily molecule is a cyanine dye, and it has no mutagenicity at the gel staining concentration. It is safe to use and sensitive in detection. It can be used as a stain for various nucleic acid electrophoresis and is suitable for staining with various fragment sizes. It is perfectly compatible with standard gel imaging systems and gel observation devices excited by visible light, and is suitable for ultraviolet gel imaging systems or gel observation devices excited by blue visible light. Features: 1. Safe and non-toxic: Although it can penetrate the cell membrane and enter the cell, it is a cyanine dye and has no mutagenicity at the gel staining concentration. 2. High sensitivity: suitable for electrophoretic staining of fragments of various sizes, without any influence on nucleic acid migration. 3. High stability: suitable for the preparation of agarose gel using microwave or other heating methods; it is extremely stable in acid or alkali buffer at room temperature and has strong light resistance. 4. High signal-to-noise ratio: the sample has strong fluorescence signal and low background signal. 5. Simple operation: it does not degrade during the precast gel and electrophoresis process, and can be directly observed with a visible light gel transmission instrument. 6. Wide range of applications: you can choose staining before electrophoresis (gel staining method) or staining after electrophoresis (bubble staining method); suitable for agarose gel or polyacrylamide gel electrophoresis; can be used for dsDNA, ssDNA or RNA staining. 7. Perfect compatibility: Suitable for UV gel imaging system excited by 254nm or gel observation device excited by blue visible light. Similar to the spectrum of SYBR Green. Storage conditions: 2-8℃, dark and dry, can be stored for 12 months. Product parameter Ex(nm)?:490 ???????Em(nm)?:520 Steps Ⅰ. Agarose gel electrophoresis staining (recommended method) Add Super Safe Nucleic Acid Dye to the gel 1. Gel preparation: Prepare agarose gel according to the usual operation, add concentrated 10,000x Super Safe Nucleic Acid Dye to make the final concentration in the gel 1x (for example: prepare 50ml gel, add 5μl dye), light Shake well and pour the glue. 2. Perform electrophoresis according to the conventional method and observe the results (the dye will not affect the DNA migration!). Ⅱ. Bubble dyeing method 1. Perform electrophoresis in accordance with conventional methods. 2.?Make a 3x staining solution by diluting 10,000x Super Safe Nucleic Acid Dye stock solution approximately 3,300 times into pure water with electrophoresis solution. 3. Place the gel carefully in a suitable container, such as a polypropylene container. Slowly add enough 3x staining solution to immerse the gel. Shake and stain at room temperature for about 30 minutes. 4. In the gel imager, the observation result Note 1. Because Super Safe has good thermal stability, it can be added directly to the hot agarose solution without waiting for the solution to cool. Shake, shake or invert to ensure that the dye is well mixed. You can also choose to add Super Safe stock solution to agarose powder and running buffer, and then heat it in a microwave oven or other common methods to prepare agarose gel. Super Safe is compatible with all commonly used electrophoresis buffer solutions. 2. If the bands are always dispersed or unsatisfactory, please use bubble dyeing method to confirm whether the problem is related to the dye. If the problem persists after staining, it means that the problem has nothing to do with the dye. Please try: reduce the agarose concentration; use a longer gel; extend the gel time to ensure a clear edge; improve the sample loading technique or choose the dyeing method. 3. Super Safe has a certain affinity for glassware and non-polypropylene materials. It is recommended to use polypropylene containers during dilution, storage, and dyeing. For polyacrylamide gel, please use the foam dyeing method. |
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