重组羧肽酶B
- CAS编号: 9025-24-5
- 分子式: C31H38N4O7S
- 分子量: 610.7
- EC号: 232-788-9
- PubChem编号: 3289209
- 酶学委员会编号: EC: 3.4.17.2
规格或纯度 | ≥200 units/mg,测序级 |
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英文名称 | Recombinant Carboxypeptidase B |
别名 | 序列分析纯重组羧肽酶B |
英文别名 | 9025-24-5|4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid|DTXSID801009277|FT-0623486|FT-0771545|Carboxypeptidase B from porcine pancreas, lyophilized p |
储存温度 | -20°C储存 |
运输条件 | 超低温冰袋运输 |
产品介绍 |
产品介绍: 重组羧肽酶B专一水解蛋白质C-末端碱性氨基酸(赖氨酸、精氨酸、组氨酸),又称为肽酰-L-赖氨酸(L-精氨酸)水解酶;精蛋白酶。分子量为35kD,等电点为6.0,最适pH为7-9。活性受精氨酸、赖氨酸的竞争性抑制,金属离子螯合剂如EDTA等抑制酶活。 蛋白质C-末端碱性氨基酸如精氨酸、赖氨酸和组氨酸的测定。抗体碱性峰的检测。 定义:25℃,pH7.6,1min 催化 1μmol 马脲酰-L-精氨酸水解的酶量为一个酶活单位。 Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation. |
EC号 | 232-788-9 |
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酶学委员会编号 | EC: 3.4.17.2 |
IUPAC Name | 4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid |
INCHI | InChI=1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38) |
InChi Key | TWURVFFNODFJBJ-UHFFFAOYSA-N |
Canonical SMILES | CC(C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(CC2=CC=CC=C2)C(=O)SCC3=CC=CC=C3)NC(=O)CCC(=O)O |
WGK Germany | 3 |
PubChem CID | 3289209 |
分子量 | 610.7 |
象形图 |
Health Hazard Harmful |
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信号词 | Danger |
危险声明 |
H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation H334: May cause allergy or asthma symptoms or breathing difficulties if inhaled |
预防措施声明 | P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P284,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319,P342+P316 |
WGK Germany | 3 |
个人防护装备 | Eyeshields, Gloves |