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Recombinant Human EGF Protein (FITC)(Active)

Recombinant Human EGF Protein (FITC)
规格或纯度: EGF-FITC, Active, Pichia pastoris, No tag, 971-1021aa
  • 蛋白长度: Full length protein
  • 蛋白标签: No tag

  • 应用: Flow,IF
有货

库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp170192-20μg 20μg 现货 Stock Image

基本描述

产品名称 Recombinant Human EGF Protein (FITC)
规格或纯度 EGF-FITC, Active, Pichia pastoris, No tag, 971-1021aa
产品介绍


Introduction:
Epidermal growth factor (EGF) is a hormone that stimulates division of epidermal and other cells. EGF (FITC) conjugate, which remains fully active after conjugation and can be used to study EGF receptor in cells. Fluorescently labeled EGF has enabled researchers to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more.
Conjugation:
The EGF was conjugated with FITC under optimum conditions, and unreacted FITC was removed. The number of fluorophore molecules on each EGF molecule is 1.0.
Recommended Usage:
Fluorescence is typically monitored using a flow cytometer, fluorescence microscope, or fluorimeter. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.5μg per million cells in 100 μl staining volume. It is recommended that the reagent be titrated for optimal performance for different cell lines.

生物活性 Binding EGFR
种属 Human
氨基酸 971-1021 aa
序列 NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWE
蛋白标签 No tag
蛋白长度 Full length protein
Carrier Free No
AnimalFree No
偶联标记 FITC. Ex: 493nm, Em: 528nm
来源 Recombinant

产品规格参数

应用 Flow,IF
物理外观 Lyophilized
储存缓冲液 Lyophilized from PBS (pH 7.4) buffer containing 1% BSA
防腐剂 No
复溶 This product should be reconstituted in 100μL of deionized water to make a 0.2mg/mL stock solution. Prepare working dilution on day of use. Stock solution is stable for about 8 weeks at 2-8°C as an undiluted liquid. Avoid freeze-thaw cycles. Protect fluor
储存温度 避光,-20°C储存
运输条件 超低温冰袋运输
稳定性与储存 The lyophilized products should be stored at –20°C until use. Allow the product to warm to room temperature before opening. Protect from light. When stored properly, the products should be stable for at least 12 months.

图片

Recombinant Human EGF Protein (FITC) (rp170192) - Flow Cytometry
Flow analysis of EGF receptors (red line) in A431 Cells. Cells were labeled with Recombinant Human EGF Protein (FITC) (rp170192). Unlabeled A431 Cells (black line) were used as a negative control.

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