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蛋白质和抗体
重组蛋白
细胞因子和生长因子
Recombinant Human EGF Protein(Active)

Recombinant Human EGF Protein(Active)

规格或纯度: GMP, ≥95% (SDS-PAGE), Active, Pichia pastoris, No tag, 971-1023 aa

蛋白长度: Full length protein
蛋白标签: No tag

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库存信息

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货号 (SKU)	包装规格	是否现货
rp155969-50μg	50μg	期货
rp155969-1mg	1mg	期货
rp155969-200μg	200μg	现货

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基本描述

产品名称	Recombinant Human EGF Protein
规格或纯度	GMP, ≥95% (SDS-PAGE), Active, Pichia pastoris, No tag, 971-1023 aa
产品介绍	pH值：6.5-7.5 人表皮生长因子（EGF）分子量为6.5kD，含有53个氨基酸，分子内有3个二硫键。EGF与位于细胞表面的特异受体EGFR 结合后，引起细胞内一系列的生化反应：提高细胞内的钙离子浓度水平，促进糖酵解的进行，增加蛋白质的合成，并且可以增强EGFR这一类特定基因的表达来促进DNA 的合成和细胞的增殖。 Purity ≥95% SDS-PAGE Additional sequence information Mature chain Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). Post-translational O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). pH value: 6.5-7.5 Human epidermal cell growth factor, Mannitol, Sodium dihydrogen monohydrate phosphate, Disodium hydrogen dodecahydrate phosphate, Water?
生物活性	Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED?? for this effect is typically <1 ng/mL.
种属	Human
氨基酸	971-1023 aa
序列	NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
蛋白标签	No tag
蛋白长度	Full length protein
Carrier Free	Yes
AnimalFree	Yes
Accession#	P01133
来源	Recombinant
预测分子量	6.5kD
SDS-PAGE	7.1 kDa, under reducing conditions.

产品规格参数

物理外观	Lyophilized
复溶	Reconstitute in sterile water to a concentration of 100-200μg/ml, and further dilute it in other solvents. Upon reconstitution, the product could be stored at 4 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS编号和信息	62253-63-8

安全信息

象形图	Harmful
信号词	Warning
危险声明	H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation
预防措施声明	P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319
WGK Germany	3

图片

Protein Bioactivity: Recombinant Human EGF Protein (rp155969)

Recombinant Human EGF Protein (rp155969)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED?? for this effect is typically <1ng/mL.

SDS-PAGE: Recombinant Human EGF Protein (rp155969)

Recombinant Human EGF Protein (rp155969)-SDS-PAGE
5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 7.1 kDa.