| 产品名称 | Recombinant Human Fibronectin Protein |
|---|---|
| 规格或纯度 | GMP, ≥95% (SDS-PAGE), Oryza sativa, No tag, 32-1908 aa |
| 产品介绍 |
产品介绍： 纤连蛋白（Fibronectin），是细胞外基质中的一种高分子量（~450kDa）糖蛋白，由2个分子质量约220kDa的亚基通过链间二硫键连接而成，广泛分布于机体组织中。在生命科学研究中，具有广泛的应用，比如作为细胞培养基质，提高细胞贴壁率，增强细胞代谢水平，缩短细胞生长时间，另外也可以直接添加至培养基中，促进细胞生长，增殖、维持良好细胞状态。? OsrhFN是一种来源于水稻胚乳细胞的重组人纤连蛋白，相比动物提取来源的纤连蛋白而言，无动物源性，无潜在病毒感染风险，更好的批次稳定性。 pH值：??6.0-8.0? 产品应用：? 细胞培养，体外诊断，实验检测，美妆等?? 使用方法 1、将OsrhFN用PBS 稀释至终参考浓度范围50-100μg/ml，混匀后，加入到孔板或培养瓶中，使OsrhFN液铺满培养瓶/板底。具体OsrhFN添加量参考表1。 2、将加有OsrhFN的培养瓶/板，4℃过夜或者37℃包被1小时； 3、吸净培养瓶/板中的OsrhFN液，收集后请置于4℃保存，1周内可用于其他培养瓶/板包被； 4、取出OsrhFN包被后的培养瓶/板，直接接种细胞即可； 5、如包被后的培养瓶/板暂不使用，请置于4℃密封保存，建议一周内用完。 B、细胞培养： 直接加入细胞培养基中，使用终浓度参考范围5-50ug/ml。 Fibronectin (FN) is a large, modular glycoprotein that generates a polymeric fibrillar network in the extracellular matrix (ECM), and forms soluble, disulfide-linked dimeric protomers in plasma and other body fluids. Fibronectin is a ligand for many molecules, including fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrins. It is involved in multiple cellular processes such as cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrillar network. Conversion of soluble Fibronectin to Fibronectin fibrils in the ECM is initiated by binding to cell surface integrins, resulting in exposure of cryptic epitopes necessary for polymerization. Fibronectin is made up of three types of homologous structural motifs termed FN type I, type II, and type III repeats. Alternative splicing generates multiple isoforms of Fibronectin which may have insertions of extra type III domains (EDA and EDB) or alteration of the type III connecting segment (IIICS). Differential splicing within the IIICS domain determines the presence of CS1 and CS2 sequences, and its sensitivity to proteases. The tilt angle between type III domains #9 and #10 (which contains an RGD motif) determines integrin binding affinity, suggesting how structural differences between fibrillar and soluble Fibronectin may influence their function. From the N-terminus to the furin cleavage site at amino acid 1908, human Fibronectin shares 92% amino acid sequence identity with mouse and rat Fibronectin. pH value: 6.0-8.0 |
| 种属 | Human |
| 氨基酸 | 32-1908 aa |
| 序列 | MQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGP |
| 蛋白标签 | No tag |
| Carrier Free | Yes |
| AnimalFree | Yes |
| Accession# | P02751-1 |
| 来源 | Recombinant |
| 预测分子量 | 206kD±5.5kDa |
| 应用 | SDS-PAGE, Cell culture |
|---|---|
| 物理外观 | Lyophilized |
| 复溶 | 建议用PBS重新溶解OsrhFN冻干粉，开封后尽快使用。溶解后的OsrhFN冻干粉可置于-20℃保存3-6个月，避免反复冻融。 |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Upon delivery aliquot, Store at -20°C, Avoid freeze / thaw cycles. |
| CAS编号和信息 | 86088-83-7 |
Recombinant Human Fibronectin Protein (rp155944)-SDS-PAGE
3μg/lane of Recombinant Human Fibronectin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a band at 206 kDa.
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