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Recombinant Human SARS-CoV-2 Spike Protein RBD

规格或纯度: Animal Free, >95% (SDS-PAGE), 293, C-His tag, 319-541 aa
  • 蛋白标签: C-His tag
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货号 (SKU) 包装规格 是否现货 价格 数量
rp155929-10μg 10μg 期货 Stock Image
rp155929-100μg 100μg 期货 Stock Image
rp155929-1mg 1mg 期货 Stock Image
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基本描述

产品名称 Recombinant Human SARS-CoV-2 Spike Protein RBD
规格或纯度 Animal Free, >95% (SDS-PAGE), 293, C-His tag, 319-541 aa
产品介绍


Purity:>95%, by SDS-PAGE visualized with Coomassie? Blue Staining.
Description：
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
种属 SARS-CoV-2
氨基酸 319-541 aa
序列 MRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFENLYFQGHHHHHH
蛋白标签 C-His tag
预测分子量 26.9 kDa
SDS-PAGE 36.4 kDa, reducing conditions; 34.2 kDa, non-reducing conditions

产品规格参数

物理外观 Liquid
储存缓冲液 10mM PBS，pH 7.4
浓度 Lot by Lot
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at -20°C for 1 year, store at 2-8℃ for 1 month. Avoid freeze / thaw cycle.

图片

Recombinant Human SARS-CoV-2 Spike Protein RBD (rp155929) - SDS-PAGE
3 μg/lane of Recombinant Human SARS-CoV-2 Spike Protein RBD was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining. Showing a band at 36.4?kDa under reducing conditions and 34.2?kDa under non-reducing conditions.

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