| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp154759-20μg | 20μg |
现货  |
| |
| rp154759-100μg | 100μg |
现货 |
| |
| rp154759-1mg | 1mg |
期货 |
|
| 产品名称 | Recombinant Mouse TNF-alpha Protein |
|---|---|
| 规格或纯度 | Animal Free, >97% (SDS-PAGE; HPLC ), Active, E.coli, No tag, 80-235aa |
| 产品介绍 |
肿瘤坏死因子α (TNF-α)，又称Cachectin，是肿瘤坏死因子家族中最知名的成员，可导致细胞死亡。该蛋白由中性粒细胞、活化淋巴细胞、巨噬细胞、NK细胞、LAK细胞、星形胶质细胞、内皮细胞、平滑肌细胞和一些转化细胞产生。TNF-α的自然形式是糖基化的，但非糖基化的重组TNF-α具有类似的生物活性。人和鼠TNF-α在氨基酸水平上显示了约79%的同源性，并在两种物种之间显示了交叉反应性。已经描述了TNF-α的两种受体类型，几乎所有研究的细胞类型都显示存在一种或两种受体类型。重组小鼠TNF-α是一条含有157个氨基酸的非糖基化多肽链 。 Purity >97% (SDS-PAGE; HPLC). Purity is greater than 97% as determined by SEC-HPLC and reducing SDS-PAGE. Additional sequence information NP_038721. Mature protein; soluble form. Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Post-translational The soluble form derives from the membrane form by proteolytic processing. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Tumor necrosis factor alpha (TNF-α), also called Cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. Human and murine TNF-α show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types. The recombinant Murine TNF-α is a non-glycosylated polypeptide chain containing 157 amino acids |
| 生物活性 | Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The ED?? for this effect is typically <0.1 ng/mL. |
| 种属 | Mouse |
| 氨基酸 | 80-235aa |
| 序列 | MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYL VYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPI YLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL |
| 蛋白标签 | No tag |
| 蛋白长度 | Protein fragment |
| Carrier Free | Yes |
| AnimalFree | Yes |
| Accession# | P06804 |
| 来源 | Recombinant |
| 预测分子量 | 17.4 kDa |
| 物理外观 | Lyophilized |
|---|---|
| 储存缓冲液 | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 |
| 复溶 | 1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. 2. Reconstitute in a sterile aqueous buffer to an appropriate concentration。 Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. F |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Upon delivery aliquot, Store at -20°C, Avoid freeze / thaw cycles. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution. |
Recombinant Mouse TNF-alpha Protein (rp154759)-Protein Bioactivity
Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D (Catalog # A113142). The EC?? for this effect is typically <0.1ng/mL.
Recombinant Mouse TNF-alpha Protein (rp154759)-SDS-PAGE
3μg/lane of Recombinant Mouse TNF-alpha was resolved with SDS-PAGE under reducing (R) conditions and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 16.3 kDa.
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