| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp154118-10μg | 10μg |
现货  |
| |
| rp154118-50μg | 50μg |
现货 |
| |
| rp154118-100μg | 100μg |
现货 |
| |
| rp154118-1mg | 1mg |
期货 |
|
| 产品名称 | Recombinant Mouse IL-1 beta Protein |
|---|---|
| 规格或纯度 | Animal Free, >95%(SDS-PAGE), Active, E.coli, His tag, 118-269 aa |
| 产品介绍 |
Background： IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta. Post-translational modifications： Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. |
| 生物活性 | Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED?? for this effect is typically 10-50 pg/mL. |
| 种属 | Mouse |
| 氨基酸 | 118-269 aa |
| 序列 | MVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSSHHHHHH |
| 蛋白标签 | 6×His tag at the C-terminus |
| N-末端序列 | Met |
| 蛋白长度 | Full length protein |
| Carrier Free | Yes |
| AnimalFree | Yes |
| 偶联标记 | No |
| Accession# | P10749 |
| 来源 | Recombinant |
| 预测分子量 | 18 kDa |
| SDS-PAGE | 16.1 kDa, under reducing conditions; 16.1 kDa, under non-reducing conditions. |
| 物理外观 | Lyophilized |
|---|---|
| 储存缓冲液 | PBS, pH 7.4, 5%Trehalose, 5%Mannitol |
| 复溶 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is recommended to reconstitute to a concentration of 0.1-1mg/mL.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze- |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store it under sterile conditions at -20℃~ -80℃ for more than 1 year.It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles |
Recombinant Mouse IL-1 beta Protein (rp154118)-SDS-PAGE
3μg/lane of Recombinant Mouse IL-1 beta was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 16.1 kDa.
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