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Recombinant Mouse FGF-21 Protein(Active)

功能和特点
  • 规格或纯度: Animal Free, >96%(SDS-PAGE, HPLC), Active, E.coli, No tag, 29-210 aa
  • 蛋白标签: No tag
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库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp153970-5μg (试用装)
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5μg 现货 Stock Image
rp153970-50μg
50μg 现货 Stock Image
rp153970-1mg
1mg 期货 Stock Image
rp153970-500μg
500μg 期货 Stock Image

基本描述

产品名称 Recombinant Mouse FGF-21 Protein
规格或纯度 Animal Free, >96%(SDS-PAGE, HPLC), Active, E.coli, No tag, 29-210 aa
产品介绍


Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression and the activity depends on the presence of KLB. FGF-21, in the presence of β-Klotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Murine FGF-21 shows limited binding to heparin. In addition, Murine FGF-21 respectively shows 81% and 92% a.a. identity to human and rat FGF-21, and it show activity on human and rat cells. Recombinant Murine FGF21 is a 19.9kDa globular protein containing 182 amino acid residues.

Purity

>96%(SDS-PAGE, HPLC)


Additional sequence information

A single non-glycosylated polypeptide chain containing 182 amino acids. This product is for the mature full length protein. The signal peptide is not included.


Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

种属 Mouse
氨基酸 29 to 210
序列 AYPIPDSSPLLQFGGQVRQRYLYTDDDQDTEAHLEIREDGTVVGAAHRS PESLLELKALKPGVIQILGVKASRFLCQQPDGALYGSPHFDPEACSFREL LLEDGYNVYQSEAHGLPLRLPQKDSPNQDATSWGPVRFLPMPGLLHEP QDQAGFLPPEPPDVGSSDPLSMVEPLQGRSPSYAS
纯度 >96%(SDS-PAGE, HPLC)
蛋白标签 No tag
无载体 Yes
无动物源 Yes
Accession # Q9JJN1
预测分子量 19.9 kDa

产品规格参数

物理外观 Lyophilized
储存缓冲液 Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB 500mM NaCl, pH7.4.
复溶 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. pH: 7.40 Constituent: 100% PBS Lyophilized from a 0.2 μm filtered concentrated solution in 3 脳 PBS. This product is an active

图片

Recombinant Mouse FGF-21 Protein (rp153970) - Protein Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/mL, corresponding to a specific activity of > 2.0 X 103 IU/mg in the presence of 5.0 μg/mL of rMuβ-Klotho and 10.0 μg/mL of heparin.

Recombinant Mouse FGF-21 Protein (rp153970) - SDS-PAGE
3 μg/lane of Recombinant Mouse FGF-21 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a band at 21.1 kDa.

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