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Recombinant Human PLA2G7 Protein(Active)

功能和特点
  • 规格或纯度: Animal Free, >90% (SDS-PAGE), Active, HEK293, C-His tag, 22-441 aa
  • 生物活性: Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2- Thio-PAF), in the presence of 5, 5’Dithiobis(2-nitrobenzoic acid) (DTNB). The specific activity is >5000 pmoles/min/
  • 蛋白标签: C-His tag
  • 应用: Active,SDS-PAGE
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库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp150291-10μg (试用装)
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10μg 现货 Stock Image
rp150291-50μg
50μg 现货 Stock Image
rp150291-100μg
100μg 期货 Stock Image
rp150291-1mg
1mg 期货 Stock Image

基本描述

产品名称 Recombinant Human PLA2G7 Protein
规格或纯度 Animal Free, >90% (SDS-PAGE), Active, HEK293, C-His tag, 22-441 aa
产品介绍


Purity

>90% (SDS-PAGE)?

Function

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

生物活性 Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2- Thio-PAF), in the presence of 5, 5’Dithiobis(2-nitrobenzoic acid) (DTNB). The specific activity is >5000 pmoles/min/
种属 Human
氨基酸 22-441 aa
序列 FDWQYINPVAHMKSSAWVNKIQVLMAAASFGQTKIPRGNGPYSVGCTDLMFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGG
纯度 >90% (SDS-PAGE)
蛋白标签 C-His tag
蛋白长度 Full length protein
无动物源 Yes
来源 Recombinant
预测分子量 49.2 kDa
SDS-PAGE 50-55 kDa, under reducing conditions

产品规格参数

应用 Active,SDS-PAGE
物理外观 Lyophilized
储存缓冲液 Lyophilized from sterile 50mM NaAc, 150mM NaCl, 10% Glycerol, 5% Trehalose, 5% Mannitol, 0.01% Tween-80, pH5.0
复溶 It is recommended that sterile water be added to the vial to prepare a stock solution of 0.27mg/mL.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store it under sterile conditions at -20℃ to -80℃ upon receiving. Upon delivery aliquot. Avoid freeze/thaw cycle.

图片

Recombinant Human PLA2G7 Protein (rp150291) - Enzyme Activity
Measured by its ability to cleave a colorimetric peptide substrate, 1-O-hexadecyl-2-deoxy-2-thio-S-acetyl-sn-glyceryl-3-phosphorylcholine (2-Thio-PAF) (T334670), in the presence of 5,5’Dithio-bis(2-nitrobenzoic acid) (DTNB) (D425581). The specific activity is >5,000 pmol/min/ug, as measured under the described condition.

Recombinant Human PLA2G7 Protein (rp150291) - SDS-PAGE
3 μg/lane of Recombinant Human PLA2G7 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a band at 54.2 kDa.

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