| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp148245-100μg | 100μg |
现货  |
| |
| rp148245-1mg | 1mg |
现货 |
| |
| rp148245-200μg | 200μg |
现货 |
| |
| rp148245-5mg | 5mg |
期货 |
|
| 产品名称 | Recombinant Human Leptin Protein |
|---|---|
| 规格或纯度 | Animal Free, >97%(SDS-PAGE and HPLC), Active, E.coli, No tag, 22-167 aa |
| 产品介绍 |
Purity >97% SDS-PAGE. Recombinant human Leptin was overexpressed as insoluble protein aggregate in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Function May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. |
| 生物活性 | Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| 种属 | Human |
| 氨基酸 | 22-167 aa |
| 序列 | VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC |
| 蛋白标签 | No tag |
| 蛋白长度 | Protein Fragment |
| Carrier Free | Yes |
| AnimalFree | Yes |
| Accession# | P41159 |
| 来源 | Recombinant |
| 预测分子量 | 16 kDa |
| 物理外观 | Lyophilized |
|---|---|
| 储存缓冲液 | Lyophilized from a 0.2 μm filtered concentrated solution in 50 mM PB, pH 3.5, with 0.02 % Tween-20. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst |
Recombinant Human Leptin Protein (rp148245)-Protein Bioactivity
Fully biologically active when compared to standard. The ED???as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0×10??IU/mg
Recombinant Human Leptin Protein (rp148245)-SDS-PAGE
Recombinant Human Leptin Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie? Blue staining, showing a single band at 16.0 kDa.
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