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Recombinant Human HBEGF/DTR Protein(Active)

规格或纯度: Animal Free, >97% (SDS-PAGE, HPLC), Active, E.coli, No tag, 63-148 aa
  • 蛋白长度: Full length protein
  • 蛋白标签: No tag

  • 应用: Cell Culture,Active,SDS-PAGE,HPLC
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货号 (SKU) 包装规格 是否现货 价格 数量
rp146797-10μg 10μg 现货 Stock Image
rp146797-50μg 50μg 现货 Stock Image
rp146797-100μg 100μg 现货 Stock Image
rp146797-1mg 1mg 期货 Stock Image
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基本描述

产品名称 Recombinant Human HBEGF/DTR Protein
规格或纯度 Animal Free, >97% (SDS-PAGE, HPLC), Active, E.coli, No tag, 63-148 aa
产品介绍


Purity
>97% by SDS-PAGE and HPLC analyses.

Function
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Background:
Human HB-EGF (Heparin-Binding EGF-like growth factor) is a 12-16 kDa member of the EGF family of peptide growth factors (1-3). Also known as the DTR (diphtheria toxin receptor), it is further classified as a group 2 ErbB ligand based on its ability to activate both the EGF/ErbB1 and ErbB4 receptors (4, 5). HB-EGF is synthesized as a 208 amino acid (aa) type I transmembrane preproprecursor (1, 6). It contains a 19 aa signal sequence, a 43 aa prosegment, an 86 aa mature region (aa 63-148), an 11 aa juxtamembrane cleavage peptide, a 24 aa transmembrane segment, and a 25 aa cytoplasmic tail (aa 184-208). As an integral membrane protein, HB-EGF is expressed as a 19-27 kDa protein in mammalian cells (7-9). The variability in molecular weight (MW) is attributed to heterogeneity in glycosylation and/or the utilization of multiple proteolytic cleavage sites during maturation. Mature HB-EGF is a soluble peptide that arises from proteolytic processing of the transmembrane form. It possesses an EGF-like domain between aa 104-144, and a heparin-binding motif between aa 93?113. Although the aa range for "mature" HB-EGF is typically stated to be Asp63-Leu148, potential N-terminal start (cleavage) sites also exist at Gly32, Arg73, Val74, Ser77 and Ala82 (8, 10-12). Thus, differential processing (in part) likely accounts for the 16-23 kDa range in MW noted for mammalian-derived mature HB-EGF. Proteases suggested to contribute to HB-EGF processing include TACE, MMP-3 and -7, ADAM-17 and ADAM-12 (11, 13-16). When expressed recombinantly in E.coli, HB-EGF (aa 73-148) runs at 14 kDa in SDS-PAGE; when expressed in Baculovirus, HB-EGF (aa 63-148, 77-148 and 32-148) runs at 18 kDa, 15 kDa, and 19 kDa respectively (8, 12, 17). Over aa 63-148, human HB-EGF- shares 76% and 73% aa sequence identity with rat and mouse HB-EGF, respectively (1, 18). Cells known to express HB-EGF include bronchial epithelium (19), visceral and vascular smooth muscle (20, 21), CD4+ T cells (22), cardiac muscle (23), glomerular podocytes (24), keratinocytes (13) and IL-10-secreting regulatory macrophages (25). As noted earlier, HB-EGF is known to bind to both 170 kDa EGFR and 180 kDa ErbB4, and through heterodimerization, ErbB2 (13, 26). Activity associated with ErbB4 binding appears to be limited to non-mitogenic actions, while EGFR binding induces both mitogenic and non-mitogenic activity.
生物活性 Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0×106 IU/mg.
种属 Human
氨基酸 63-148 aa
序列 DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
蛋白标签 No tag
蛋白长度 Full length protein
Carrier Free Yes
AnimalFree Yes
Accession# Q99075
来源 Recombinant
预测分子量 9.7 kDa
SDS-PAGE 14.4 kDa, under reducing conditions

产品规格参数

应用 Cell Culture,Active,SDS-PAGE,HPLC
物理外观 Lyophilized
储存缓冲液 Lyophililzed from 20 mM PB, pH 7.4, 130 mM NaCl
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1 mg/ml. Stock solutions should be apportioned
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

图片

Recombinant Human HBEGF/DTR Protein (rp146797)-SDS-PAGE
Recombinant Human HBEGF/DTR Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie? Blue staining. Showing a single band at 14.4?kDa.

Recombinant Human HBEGF/DTR Protein (rp146797) - Protein Bioactivity
Fully biologically active when compared to standard. The ED50?as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 10??U/mg.

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