| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp145939-50μg | 50μg |
现货  |
| |
| rp145939-100μg | 100μg |
现货 |
| |
| rp145939-1mg | 1mg |
现货 |
| |
| rp145939-500μg | 500μg |
现货 |
|
| 产品名称 | Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein |
|---|---|
| 规格或纯度 | GMP, >95% SDS-PAGE, Active, E.coli, No tag, 134-288 aa |
| 产品介绍 |
重组人碱性成纤维细胞生长因子 (又称为碱性 FGF、bFGF、FGF2、FGF-β或者肝素结合生长因子)，是一种适 用于细胞培养应用的生物活 性蛋白质。bFGF 可调节细胞增殖、分化、存活、粘附、运动、凋亡、肢体形成和伤口恢复等多种过程。bFGF 可用于血管发生、成纤维细胞有丝分列、PC-12 细胞的轴突生长、受体结合以及酪氨 酸磷酸化研究。 本品系重组大肠杆菌表达、经多步分离纯化，溶解于 10mM PBS，0.15 M NaCl（pH7.2）溶液中，再经 0.22 μm 滤膜过滤除.菌后冷冻干燥而制成的冻干粉。 Purity >95% SDS-PAGE. lyophilised from 0.22 μm filtered solution. Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). Post-translational Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-beta, or heparin-binding growth factor), is a biologically active protein suitable for cell culture applications. bFGF regulates diverse processes such as cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation, and wound recovery. bFGF can be used in studies of angiogenesis, fibroblast mitosis, axonal outgrowth in PC-12 cells, receptor binding, and tyrosine phosphorylation. This strain is expressed in recombinant Escherichia coli, and after multi-step separation and purification, it is dissolved in 10mM PBS, 0.15 M NaCl (pH7.2) solution, filtered through a 0.22 μm filter membrane, and then freeze-dried to make a lyophilized powder. . |
| 生物活性 | Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED?? for this effect is typically <0.9 ng/mL. |
| 种属 | Human |
| 氨基酸 | 134-288 |
| 序列 | MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS |
| 蛋白标签 | No tag |
| 蛋白长度 | Full length protein |
| Carrier Free | Yes |
| AnimalFree | Yes |
| Accession# | P09038 |
| 来源 | Recombinant |
| 预测分子量 | 17 kDa |
| 物理外观 | Lyophilized |
|---|---|
| 储存缓冲液 | 10mM PBS |
| 复溶 | 建议开盖前短暂离心使粉末沉于管底，然后用灭菌过的去离子水溶解，配制为 100μg/ml 的储存液，2℃ ~8℃可稳定保存一周。稀释到较低浓度(不低于 10μg/ml)时，需要加入 0.1%的组织培养级 BSA 或 HSA。 若需延长储存时间，建议在-80℃或-20℃冷冻，不建议重复冷冻和解冻。 |
| 储存温度 | -80℃储存,避免反复冻融 |
| 运输条件 | 冰袋运输 |
| 稳定性与储存 | 在-15℃及以下稳定至 24 个月，在 2~8℃下稳定至 4 周。配制为 100μg/ml 的储存液，2~8℃ 可稳定保存一周。 |
Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-SDS-PAGE
2μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 15.6 kDa.
Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED?? for this effect is typically <0.9ng/mL.
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