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研究领域
肿瘤学
生长因子
表皮生长因子(EGF)
Recombinant Human EGF Protein(Active)

Recombinant Human EGF Protein(Active)

规格或纯度: GMP, >95% SDS-PAGE, Active,Pichia pastoris, No tag, 971-1021aa

纯度: ＞95% SDS-PAGE
蛋白长度: Full length protein

蛋白标签: No tag

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货号 (SKU)	包装规格	是否现货
rp145392-100μg	100μg	现货
rp145392-1mg	1mg	现货
rp145392-500μg	500μg	现货

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基本描述

产品名称	Recombinant Human EGF Protein
规格或纯度	GMP, >95% SDS-PAGE, Active,Pichia pastoris, No tag, 971-1021aa
产品介绍	Purity ≥ 95% SDS-PAGE. ≥ 95 % HPLC. Additional sequence information N-terminal glycine. Full-length mature EGF chain. Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). Post-translational O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
生物活性	Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED?? for this effect is typically <0.9 ng/mL.
种属	Human
氨基酸	971-1021 aa
序列	NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWW ELR
蛋白标签	No tag
N-末端序列	NSDSECPLSH
蛋白长度	Full length protein
Carrier Free	Yes
AnimalFree	Yes
Accession#	P01133
来源	Recombinant
预测分子量	6 kDa

产品规格参数

物理外观	Lyophilized
储存缓冲液	10mM磷酸钠缓冲液
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 10mM PBS to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	在-20℃下稳定至 24 个月，在 2~8℃下稳定至 4 周。配制为 100μg/ml 的储存液，2~8℃可稳定保存一周。若需延长储存时间，建议在-70℃或-20℃冷冻，不建议重复冷冻和解冻。

图片

Protein Bioactivity: Recombinant Human EGF Protein (rp145392)

Recombinant Human EGF Protein (rp145392)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED?? for this effect is typically <0.9ng/mL.

SDS-PAGE: Recombinant Human EGF Protein (rp145392)

Recombinant Human EGF Protein (rp145392)-SDS-PAGE
5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 6.6 kDa.