| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp143202-10μg | 10μg |
现货  |
| |
| rp143202-50μg | 50μg |
现货 |
| |
| rp143202-100μg | 100μg |
现货 |
| |
| rp143202-1mg | 1mg |
期货 |
|
| 产品名称 | Recombinant Human Beta-Defensin 3 Protein |
|---|---|
| 规格或纯度 | Animal Free, >98%(SDS-PAGE, HPLC), Active, E.coli, No tag, 23-67 aa |
| 产品介绍 |
Purity > 98% by SDS-PAGE and HPLC analyses. Function Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observe. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. |
| 生物活性 | Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 μg/ml. |
| 种属 | Human |
| 氨基酸 | 23-67 aa |
| 序列 | GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK |
| 蛋白标签 | No tag |
| 蛋白长度 | Full length protein |
| Carrier Free | Yes |
| AnimalFree | Yes |
| Accession# | P81534 |
| 来源 | Recombinant |
| 预测分子量 | 5.2 kDa |
| 应用 | Active, SDS-PAGE |
|---|---|
| 物理外观 | Lyophilized |
| 储存缓冲液 | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ℃ as supplied; 1 month, 2 to 8 ℃ under sterile conditions after reconstitution; 3 months, -20 to -70 ℃ under sterile conditions after reconstitu |
Recombinant Human Beta-Defensin 3 Protein (rp143202)-Protein Bioactivity
Fully biologically active when compared to standard. The ED???as determined by anti-microbial activity against E.coli. is less than 30 μg/mL, corresponding to a specific activity of > 33.3 IU/mg.
Recombinant Human Beta-Defensin 3 Protein (rp143202)-SDS-PAGE
Recombinant Human Beta-Defensin 3 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie? Blue staining, showing a single band at 5.2 kDa.
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