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RTGreen核酸染料
规格或纯度:
20× in water
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基本描述
| 规格或纯度 | 20× in water |
|---|---|
| 英文名称 | RTGreen nucleic acid dye |
| 储存温度 | 2-8°C储存,避光,干燥 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
产品介绍 RTGreen?是一种用于实时定量?PCR（qPCR）的DNA结合染料。该染料的诸多优点使它远胜于EvaGreen和Sybr Green。除了有相似的光谱特性，RTGreen有三个主要特点使它区别于SYBR Green I。 1.??RTGreen对PCR的抑制性远小于SYBR Green I。因此，使用RTGreen进行的qPCR实验可以使用快速PCR步骤。同时，RTGreen在实验中可以使用较高的浓度，从而获得远强于SYBR Green I扩增信号。较高浓度的RTGreen也消除了“染料重分布”的缺陷，使RTGreen既可用于多重PCR，也可用于高分辨率（高清晰）熔解曲线分析（HRM）。该分析正被越来越多的用于PCR后的基因分型和异源双链分析。由于SYBR Green I对PCR的抑制性，从而要求其使用浓度必须很低，因此?SYBR Green I无法解决由低浓度造成的染料重分布问题，既不能用于多重PCR也不能用于HRM。同时，染料重分布问题也可能影响常规熔解曲线的可靠性，因为低熔点的DNA链可能由于这种原因而无法检测到?。 2.??RTGreen的稳定性极强。在正常的储存、操作和PCR过程中不会被破坏。在缓冲溶液中的染料可以安全的储存在室温或冰箱里，也可以反复冻融。与之相反，SYBR Green I不稳定而且降解后对PCR抑制性更强?。 3.??RTGreen降低了细胞膜透性，因而比SYBR Green I更加安全。独立实验室的测试结果显示，RTGreen既没有诱变性也没有细胞毒性。相反，虽然?SYBR Green I本身诱变性很弱，但它在细胞中可能抑制了正常DNA的修复机制，使其有诱变增强作用。 4. Ex(nm)：495，Em(nm)：520 ? 产品特性 极高的灵敏度 在推荐浓度下1X使用时可以获得最强的PCR扩增信号。 PCR?抑制性极小 智能化的“按要求释放?”DNA结合技术使得RTGreen对PCR的抑制远小于SYBR Green I。 和快速PCR兼容 ?对PCR干扰极小，从而极大的缩短了PCR延伸时间。 非常适合HRM分析 无“染料重分布”缺陷，兼容PCR后的高分辨率熔解曲线（HRM）分析。 兼容多重?PCR 在推荐浓度下使用时，无扩增子之间的染料迁移现象。 超强稳定性 在大部分生化条件下非常稳定，可在室温下储存并可反复冻融。 安全性好 细胞膜穿透性测试表明，RTGreen几乎不能穿透细胞膜，安全性高。 优越的兼容性 和EvaGreen,Sybr Greeb光谱相似,和各知名品牌的qPCR仪器兼容。?用RTGreen?替代EvaGreen或者Sybr Green，毋须改变任何您目前使用的操作步骤和仪器设备。 Product description RTGreen is a DNA binding dye used for real-time quantitative PCR (qPCR). The many advantages of this dye make it far superior to EvaGreen and Sybr Green. In addition to having similar spectral characteristics, RTGreen has three main characteristics that distinguish it from SYBR Green I. 1. The inhibitory effect of RTGreen on PCR is much less than that of SYBR Green I. Therefore, qPCR experiments using RTGreen can use a fast PCR step. At the same time, RTGreen can use a higher concentration in the experiment, so as to obtain a much stronger amplification signal than SYBR Green I. The higher concentration of RTGreen also eliminates the defect of "dye redistribution", making RTGreen not only used for multiplex PCR, but also for high resolution (high definition) melting curve analysis (HRM). This analysis is increasingly being used for genotyping and heteroduplex analysis after PCR. Due to the inhibitory properties of SYBR Green I on PCR, the use concentration must be very low. Therefore, SYBR Green I cannot solve the problem of dye redistribution caused by low concentration, and it cannot be used for multiplex PCR or HRM. At the same time, the problem of dye redistribution may also affect the reliability of conventional melting curves, because DNA strands with low melting points may not be detected for this reason. 2. RTGreen is extremely stable. It will not be damaged during normal storage, operation and PCR. The dye in the buffer solution can be safely stored at room temperature or in the refrigerator, or it can be repeatedly frozen and thawed. In contrast, SYBR Green I is unstable and has a stronger inhibitory effect on PCR after degradation. 3. RTGreen reduces cell membrane permeability, so it is safer than SYBR Green I. Independent laboratory test results show that RTGreen is neither mutagenic nor cytotoxic. On the contrary, although SYBR Green I itself is very weakly mutagenic, it may inhibit the normal DNA repair mechanism in cells, making it mutagenic and enhancing. 4. Ex(nm)：495，Em(nm)：520 Product Features Very high sensitivity The strongest PCR amplification signal can be obtained when 1X is used at the recommended concentration. PCR inhibition is minimal The intelligent "release on demand" DNA binding technology makes RTGreen's inhibition of PCR far less than SYBR Green I. Compatible with fast PCR It has minimal interference to PCR, which greatly reduces the PCR extension time. Ideal for HRM analysis No "dye redistribution" defect, compatible with high resolution melting curve (HRM) analysis after PCR. Compatible with multiple PCR When used at the recommended concentration, there is no dye migration between amplicons. Super stability It is very stable under most biochemical conditions, can be stored at room temperature and can be frozen and thawed repeatedly. Good security The cell membrane penetration test shows that RTGreen can hardly penetrate the cell membrane and is highly safe. Superior compatibility Similar to EvaGreen and Sybr Greeb spectra, it is compatible with qPCR instruments of various well-known brands. Use RTGreen to replace EvaGreen or Sybr Green without changing any of your current operating procedures and equipment. |
化学和物理性质
| 敏感性 | 对光和湿度敏感 |
|---|
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产品问答
上海阿拉丁生化科技股份有限公司
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