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Polyclonal Antibody to Human Topoiosomerase I (Scleroderma Patient Serum)
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基本描述
| 英文名称 | Polyclonal Antibody to Human Topoiosomerase I (Scleroderma Patient Serum) |
|---|---|
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 产品介绍 |
Product Info Unit Definition: The Polyclonal Antibody to Human Topoiosomerase I concentration is in Western blotting units; one unit corresponds to a 1:1000 dilution of probe for a routine Western blot (1 ml).? For example, 10 units of antibody will make 10 ml of diluted probe (to be added directly to a single blot). Material Safety Data: THIS PRODUCE IS NOT LICENSED OR APPROVED FOR ADMINISTRATION TO HUMANS OR TO ANIMALS OTHER THAN EXPERIMENTAL ANIMALS. Description Some scleroderma patients produce high titer antibody directed against human topoisomerase I (Shero et al., 1986).? The immunoglobulin fraction was purified from these patients and shown to react specifically with the 100 kDa form of Topo I using Western blotting.? The immune complexes produced with this reagent will bind [125I]-protein A.? When intact topoisomerase I is used, a single polypeptide of 100 kDa was detected; however, sub-bands produced by proteolysis will also bind this reagent, as expected.? The antibody is particularly useful as a Western blotting probe and can be used at high dilutions (>1:5,000).? Cross reactivity of this antibody with other species has been demonstrated (with calf thymus).? Important note about product use: Human topoisomerase I undergoes spontaneous degradation especially with repeat freezing/thawing. The activity of the enzyme is not affected significantly; however, sub-bands may be detectable in Western blotting experiments. This antibody may be used in immunoprecipitation experiments. Shipping&storage The antibody is shipped hydrated on wet ice. Store at -20°C. |
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