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Recombinant Streptavidin Protein (APC)

Recombinant Streptavidin Protein (APC)

规格或纯度: Recombinant; Streptavidin (APC); Flow

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货	价格	数量
rp169048-100μl	100μL	现货
rp169048-1ml	1ml	现货

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基本描述

产品名称	Recombinant Streptavidin Protein (APC)
规格或纯度	Recombinant; Streptavidin (APC); Flow
产品介绍	Introduction: Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a dissociation constant on the order of ≈10?1? mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, microplate assays and microarrays. Conjugation: The APC was conjugated with Streptavidin under optimum conditions. Unconjugated streptavidin and mostly unconjugated APC were removed. The APC conjugates are prepared from chemically crosslinked APC to avoid dissociation of the molecule into subunits when highly diluted. Recommended Usage for Application: Flow: 0.02-1.25μg/mL Application Note: Centrifuge protein conjugate solutions briefly in a microcentrifuge before use and use only the supernatant for the experiment. This step will eliminate any protein aggregates that may have formed in solution, thereby reducing nonspecific background staining.

产品规格参数

物理外观	Liquid
储存缓冲液	10mM PBS (pH 7.4) buffer containing 1% BSA and 0.05% ProClin300
防腐剂	0.05% ProClin300
浓度	0.2 mg/mL
储存温度	2-8°C储存,避光
运输条件	冰袋运输
稳定性与储存	Shipped at 4℃. Store at 4°C and protected from light. Do not freeze.
CAS编号和信息	9013-20-1

安全信息

象形图	Harmful
信号词	Warning
危险声明	H315: Causes skin irritation H319: Causes serious eye irritation H335: May cause respiratory irritation
预防措施声明	P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319
WGK Germany	3
个人防护装备	Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

应用

Application	Notes
Flow	0.02-1.25 μg/mL

图片

Flow Cytometry: Recombinant Streptavidin Protein (APC) (rp169048)

Recombinant Streptavidin Protein (APC) (rp169048) - Flow Cytometry
BALB/c mouse splenocytes stained with Biotin-CD45R antibody, followed by Recombinant Streptavidin Protein (APC) (rp169048) at 0.03 μg/test concentration.

Flow Cytometry: Recombinant Streptavidin Protein (APC) (rp169048)

Recombinant Streptavidin Protein (APC) (rp169048) - Flow Cytometry
BALB/c mouse splenocytes stained with Biotin-CD45R antibody for 1 hour at 4°C, followed by 0.03 μg Recombinant Streptavidin Protein (APC) (rp169048) (red line) for 30 minutes at 4°C in the dark . Unlabeled samples (black line) were used as a negative control.